Cheng Lu

US Patent:

20060063181, Mar 23, 2006

Filed:

Aug 15, 2005

Appl. No.:

11/204903

Inventors:

Pamela Green - Newark DE, US
Blake Mayers - Wilmington DE, US
Cheng Lu - Newark DE, US
Christian Haudenschild - San Francisco CA, US
Shujun Luo - Castro Valley CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

A method of identifying and quantifying small RNA molecules comprising a) isolating RNA molecules; b) ligating RNA adapter molecules onto the isolated RNA molecules to form RNA template molecules; c) forming complementary DNA molecules by transcribing the RNA template molecules; d) amplifying the complementary DNA molecules; e) obtaining sequence information of the complementary DNA molecules (and thereby the RNA from which it was derived); and f) obtaining quantity information of the complementary DNA molecules, wherein the quantity information of the DNA molecules reflects the quantity of the isolated RNA molecules is provided. Included in the invention is the identification of RNA molecules between 15 and 30 nucleotides in length.

US Patent:

20070111227, May 17, 2007

Filed:

Jul 28, 2006

Appl. No.:

11/495951

Inventors:

Pamela Green - Newark DE, US
Blake Meyers - Wilmington DE, US
Cheng Lu - Newark DE, US
Shivakundan Tej - Herndon VA, US
Frederic Souret - Newark DE, US

International Classification:

C12Q 1/68
G06F 19/00
C40B 40/08

US Classification:

435006000, 702020000

Abstract:

The present invention relates to unique small ribonucleic acid molecules, for example siRNAs and miRNAs, identified and isolated using MPSS. Specifically, the invention is directed to the identification of a library of unique small RNA sequences from . In another aspect, the small RNA sequences themselves are useful for performing biological functions, such as for example, RNA interference.

US Patent:

20220298520, Sep 22, 2022

Filed:

Jun 17, 2020

Appl. No.:

17/619583

Inventors:

- Johnston IA, US
Wenpin Chen - Brampton, CA
Igor Falak - Guelph, CA
Siva S. Ammiraju JETTY - Johnston IA, US
Cheng Lu - Newark DE, US
Robert W. Williams - Minneapolis MN, US

Assignee:

PIONEER HI-BRED INTERNATIONAL, INC. - Johnston IA

International Classification:

C12N 15/82
C12N 9/22
C12N 9/24
C12Q 1/6895
C12N 15/11

Abstract:

Genome edited plants, plant cells, seeds and plant parts of are provided where expression levels and/or activities of pod dehiscence genes are modulated to improve one or more agronomic characteristics such as pod shatter. Also provided are compositions comprising polynucleotides encoding polypeptides and guide RNAs targeted to endogenous proteins involved in pod dehiscence including for example, targeted site-directed mutagenesis using CRISPR-associated nucleases. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a plant and methods for increasing pod shatter tolerance of a plant are also provided herein.

US Patent:

20210403933, Dec 30, 2021

Filed:

Nov 12, 2019

Appl. No.:

17/293532

Inventors:

- JOHNSTON IA, US
ZHAN-BIN LIU - CLIVE IA, US
CHENG LU - NEWARK DE, US
BO SHEN - JOHNSTON IA, US

Assignee:

PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA

International Classification:

C12N 15/82

Abstract:

Soybean seeds with increased protein and having a modified expression or activity of at least one or two HECT E3 ligase polypeptides are provided. Methods for modifying expression or activity of HECT E3 ligase polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the HECT E3 ligase polypeptides and transformation with recombinant DNA constructs to enhance or suppress expression or activity of the HECT E3 ligase polypeptides. Plants containing the modifications produce seeds with altered composition such as one or more of increased protein, decreased soluble carbohydrate, increased oleic acid, decreased saturated fats such as palmitic and stearic acids, and decreased linoleic or linolenic acid.

US Patent:

20170211088, Jul 27, 2017

Filed:

Apr 7, 2017

Appl. No.:

15/481594

Inventors:

- Wilmington DE, US
- Johnston IA, US
JASON L. BROTHERS - NEWARK DE, US
KRUPA DESHMUKH - HYDERABAD, IN
XIAO-YI LI - WILMINGTON DE, US
CHENG LU - NEWARK DE, US
STANLEY LUCK - WILMINGTON DE, US
JEFFREY MULLEN - MINNETRISTA MN, US
HAJIME SAKAI - NEWARK DE, US
JAMES J SAYLOR - NEWARK DE, US
SCOTT V. TINGEY - ROCKDALE TX, US
ROBERT WAYNE WILLIAMS - HOCKESSIN DE, US

International Classification:

C12N 15/82

Abstract:

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP6 polypeptide.

US Patent:

20160369295, Dec 22, 2016

Filed:

Dec 22, 2014

Appl. No.:

15/107126

Inventors:

- Wilmington DE, US
- Johnston IA, US
Norbert Brugiere - Johnston IA, US
Zhenglin Hou - Ankeny IA, US
Ratna Kumria - Hyderabad, IN
H. Renee Lafitte - Davis CA, US
Xiao-Yi Li - Wilmington DE, US
Cheng Lu - Newark DE, US
Stanley Luck - Wilmington DE, US
Amitabh Mohanty - Hyderabad, IN
Jeffery Mullen - Minnetrista MN, US
Rupa Raja - Secunderabad, IN
Hajime Sakai - Newark DE, US
Scott V. Tingey - Rockdale TX, US
Robert W. Williams - Hockessin DE, US

Assignee:

Pioneer Hi-Bred International Inc. - Johnston IA
E. I. du Pont de Nemours and Company - Wilmington DE

International Classification:

C12N 15/82
C12N 9/18

Abstract:

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring stress tolerance are presented herein, along with compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP4 polypeptide.

US Patent:

20140223595, Aug 7, 2014

Filed:

Oct 28, 2011

Appl. No.:

13/877814

Inventors:

Stephen M. Allen - Wilmington DE, US
Jason L. Brothers - Newark DE, US
Krupa Deshmukh - Hyderabad, IN
Honor Renee Lafitte - Davis CA, US
Xiao-Yi Li - Wilmington DE, US
Cheng Lu - Newark DE, US
Stanley Luck - Wilmington DE, US
Jeffrey Mullen - Newark DE, US
Hajime Sakai - Newark DE, US
James J. Saylor - Newark DE, US
Scott V. Tingey - Wilmington DE, US
Robert Wayne Williams - Hockessin DE, US

Assignee:

E I DU PONT DE NEMOURS AND COMPANY - Wilmington DE

International Classification:

C12N 15/82
G06F 19/20

US Classification:

800278, 536 236, 800298, 800300, 800306, 800312, 800314, 800320, 8003201, 8003202, 8003203, 800322, 702 19

Abstract:

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP6 polypeptide.