Richard Fike - Clarence NY, US William Whitford - South Logan UT, US Richard Hassett - Tonawanda NY, US William Biddle - Buffalo NY, US Jeffrey Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine Biddle - Buffalo NY, US Robert Radominski - Tonawanda NY, US Barbara Dadey - East Aurora NY, US
Assignee:
INVITROGEN CORPORATION - Carlsbad CA
International Classification:
B01J 8/18 C12N 5/00
US Classification:
422139000, 435405000
Abstract:
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
Dry Powder Cell Culture Products And Methods Of Production Thereof
Richard M. Fike - Clarence NY, US William Whitford - Logan UT, US Richard F. Hassett - Tonawanda NY, US Jeffrey W. Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine M. Biddle - Buffalo NY, US Robert C. Radominski - Tonawanda NY, US Barbara M. Dadey - East Aurora NY, US
Assignee:
INVITROGEN CORPORATION - Carlsbad CA
International Classification:
C12N 5/00
US Classification:
435404
Abstract:
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
Dry Powder Cell Culture Products And Methods Of Production Thereof
Richard M. Fike - Clarence NY, US William Whitford - Logan UT, US Richard F. Hassett - Tonawanda NY, US Jeffrey W. Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine M. Biddle - Buffalo NY, US Robert C. Radominski - Tonawanda NY, US Barbara M. Dadey - East Aurora NY, US
Assignee:
INVITROGEN CORPORATION - Carlsbad CA
International Classification:
C12N 5/06
US Classification:
435404
Abstract:
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
Dry Powder Cell Culture Products And Methods Of Production Thereof
Richard M. FIKE - Clarence NY, US William WHITFORD - Logan UT, US Richard F. HASSETT - Tonawanda NY, US Jeffrey W. BIDDLE - Buffalo NY, US Laurel BIDDLE - Buffalo NY, US Christine M. BIDDLE - Buffalo NY, US Robert C. RADOMINSKI - Tonawanda NY, US Barbara M. DADEY - East Aurora NY, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12N 5/071 C12N 5/04 C12N 1/16 C12N 5/00
US Classification:
435404, 435420, 4352568
Abstract:
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
Dry Powder Cell Culture Products And Methods Of Production Thereof
Richard M. Fike - Clarence NY, US William Whitford - Logan UT, US Richard F. Hassett - Tonawanda NY, US Jeffrey W. Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine M. Biddle - Buffalo NY, US Robert C. Radominski - Tonawanda NY, US Barbara M. Dadey - East Aurora NY, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12N 5/00
US Classification:
435404, 435431, 4352536, 4352568
Abstract:
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
Dry Powder Cells And Cell Culture Reagents And Methods Of Production Thereof
Richard Fike - Clarence NY, US William Whitford - Santa Fe NM, US William Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine Biddle - Buffalo NY, US Jeffrey Biddle - Buffalo NY, US Richard Hassett - Tonewanda NY, US Barbara Dadey - East Aurora NY, US
International Classification:
C12N 5/02
US Classification:
435404000
Abstract:
The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.
Dry Powder Cells And Cell Culture Reagents And Methods Of Production Thereof
Richard Fike - Clarence NY, US William Whitford - Santa Fe NM, US William Biddle - Buffalo NY, US Laurel Biddle - Buffalo NY, US Christine Biddle - Buffalo NY, US Jeffrey Biddle - Buffalo NY, US
International Classification:
C12N 5/02
US Classification:
435404000
Abstract:
The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.