Christine R Collins

US Patent:

46525297, Mar 24, 1987

Filed:

Oct 2, 1984

Appl. No.:

6/657319

Inventors:

Christine G. Collins - San Jose CA
Susan Pankey - Palo Alto CA
Anna Jaklitsch - Palo Alto CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

G01N 3002
G01N 3350

US Classification:

436 92

Abstract:

Serum is passed through a column containing alkylated silica gel. The column is then washed with a mixture comprising from about 50 to 85 volume percent of an aqueous buffered medium of pH from about 3. 5 to 5. 0 and from about 15 to 50 volume percent of an organic solvent containing from 1 to 6 carbon atoms and from 1 to 5 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur. The tricyclic antidepressant drug is removed from the column with an eluent mixture comprising from about 0 to 75 volume percent of an aqueous buffered medium of pH of about from 6 to 8 and from about 25 to 100 volume percent of one or more organic solvents containing from 1 to 6 carbon atoms and from 1 to 5 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur. In this way, a tricyclic antidepressant drug sample, free from interfering metabolites, for use in assay determinations is obtained.

US Patent:

47726970, Sep 20, 1988

Filed:

Aug 21, 1986

Appl. No.:

6/898559

Inventors:

Christine G. Collins - San Jose CA
Marcel R. Pirio - San Jose CA
Prithipal Singh - Sunnyvale CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

C07D22328

US Classification:

540591

Abstract:

Tricyclic antidepressant functionalized compounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies and together with the enzyme conjugates find particular use in immunoassays for the determination or detection of the total amount of tricyclic antidepressants present in a sample.

US Patent:

46296913, Dec 16, 1986

Filed:

Aug 12, 1983

Appl. No.:

6/522887

Inventors:

Christine G. Collins - San Jose CA
Marcel R. Pirio - San Jose CA
Prithipal Singh - Sunnyvale CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

G01N 5300
C12N 996

US Classification:

435 7

Abstract:

Tricyclic antidepressant functionalized compounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies and together with the enzyme conjugates find particular use in immunoassays for the determination or detection of the total amount of tricyclic antidepressants present in a sample.