Christine D Lambert

US Patent:

20100261229, Oct 14, 2010

Filed:

Apr 8, 2010

Appl. No.:

12/756547

Inventors:

Aldrich N. K. LAU - Palo Alto CA, US
Christine LAMBERT - San Mateo CA, US
Achim KARGER - Foster City CA, US
Madison JORDAN - Foster City CA, US

Assignee:

APPLIED BIOSYSTEMS, LLC - Carlsbad CA

International Classification:

C12P 19/34
C12M 1/00

US Classification:

435 912, 4352891

Abstract:

An emulsion generation apparatus and method for forming an emulsion are provided wherein a customized impeller design is adapted to form an emulsion with a desired droplet size that defines a desired volume. The emulsion generation apparatus provides improved uniformity in emulsion preparation and may be used to create large or small volume emulsions rapidly and reproducibly. A system and method are also provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions and other reactions. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.