Christopher Michael Earhart from San Jose, CA, age 44

Magnetic Separation Device For Cell Sorting And Analysis
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US Patent:

20110059468, Mar 10, 2011
Filed:

Sep 9, 2010
Appl. No.:

12/807625
Inventors:

Christopher M. Earhart - Palo Alto CA, US
Shan X. Wang - Portola Valley CA, US
Robert J. Wilson - Campbell CA, US
International Classification:

G01N 33/53
C12M 1/00
C12N 13/00
C12Q 1/06
US Classification:

435 721, 4352831, 4351739, 435 39
Abstract:

A magnetic sifter is adapted for manipulation of biological cells by providing a greater pore density at the edge of the sifter than at the center. Application of an external magnetic field to the sifter causes high magnetic fields and field gradients at the sifter pores. These conditions are suitable for capturing magnetically tagged or labeled cells at the sifter pores. Altering the external magnetic field can provide controlled capture and/or release of magnetically labeled cells from the sifter pores. The purpose of having a greater pore density at the periphery of the sifter than at the center is to provide improved flow rate uniformity through the sifter. Such flow rate uniformity is advantageous for cell quantification.

System And Method For Characterizing Particulates In A Fluid Sample
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US Patent:

20230090141, Mar 23, 2023
Filed:

Nov 29, 2022
Appl. No.:

18/059490
Inventors:

- Burlingame CA, US
Brian DiPaolo - Marlton NJ, US
Gjergji Konica - King of Prussia PA, US
Thomas Castner - Jenkintown PA, US
Bernardo Cordovez - San Francisco CA, US
Christopher Earhart - Philadelphia PA, US
David Erickson - Ithaca NY, US
Robert Hart - Philadelphia PA, US
International Classification:

G02B 21/36
G01N 15/02
G02B 21/24
G01N 15/14
G01N 21/64
G02B 21/12
G02B 21/16
Abstract:

A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.

Methods For Identification Of Particles In A Fluid Sample
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US Patent:

20200217795, Jul 9, 2020
Filed:

Dec 24, 2019
Appl. No.:

16/726357
Inventors:

- Philadelphia JP, US
Brian DiPaolo - Marlton NJ, US
Gjergji Konica - King of Prussia PA, US
Thomas Castner - Jenkintown PA, US
Bernardo Cordovez - San Francisco CA, US
Christopher Earhart - Philadelphia PA, US
David Erickson - Ithaca NY, US
Robert Hart - Philadelphia PA, US
International Classification:

G01N 21/64
G01N 33/68
G01N 1/30
Abstract:

A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.

System And Method For Characterizing Particulates In A Fluid Sample
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US Patent:

20170242234, Aug 24, 2017
Filed:

Feb 21, 2017
Appl. No.:

15/438092
Inventors:

- Philadelphia PA, US
Brian DiPaolo - Marlton NJ, US
Gjergji Konica - King of Prussia PA, US
Thomas Castner - Jenkintown PA, US
Bernardo Cordovez - San Francisco CA, US
Christopher Earhart - Philadelphia PA, US
David Erickson - Ithaca NY, US
Robert Hart - Philadelphia PA, US
International Classification:

G02B 21/36
G02B 21/16
G02B 21/12
G01N 21/64
G01N 15/14
Abstract:

A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.