Jeffrey L. Sklar - Chestnut Hill MA Douglas T. Gjerde - Saratoga CA Kimberly A. Lamb - Omaha NE Christopher Hanna - Somerville MA
Assignee:
Transgenomic, Inc. - San Jose CA
International Classification:
C12Q 168 C12P 1934 C07H 2104 B01D 1500
US Classification:
435 912
Abstract:
A method for detecting a putative mutant DNA in a sample of DNA includes the steps of amplifying the sample of DNA using PCR; hybridizing the amplified sample to form a mixture of homoduplexes and heteroduplexes; separating the mixture into fractions by Denaturing Matched Ion Polynucleotide Chromatography; and blind collecting the eluted fractions at a retention time corresponding to the retention time of the heteroduplex. The DNA in the blind collected fractions can be PCR amplified to obtain an increased amount of heteroduplex relative to homoduplex. The method is useful for determining the remission status of a patient in which the tissue-derived DNA sample contains a large background of wild type or where the putative mutant DNA is below the limit of detection.
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Googleplus
Christopher Hanna (Beatni...
Work:
Lots of places
Education:
Sage College of Albany - Creative Studies- Writing
About:
Www.scribd.com/chris_hanna_7
Tagline:
I'm a twenty something truth seeker, want to be enlightened wisdom drinker
Bragging Rights:
Poet. I like to mix ideas, bits of phrases I hear from conversations, thoughts or phrases I can't get out of my head from books and from song lyrics that get stuck in my brain. I am on a quest, I guess you have to call it that, to read as much as I can of all books I find that I hear are profound, seem deep in ideas, or seem rich in intrigue. I am a fountain of arbitrary arguments.
Christopher Hanna
Education:
Foothill Technology High School
About:
Hi, my name is Chris Hanna, master of all things tech and geek. I express myself through photography, and am always looking for the next thing.
Tagline:
Digital Enthusiast
Bragging Rights:
Survivor of Middle School, Bass Lake Parties, & the PSN Hack Attack