Christopher S Power

US Patent:

50305727, Jul 9, 1991

Filed:

Dec 14, 1989

Appl. No.:

7/453155

Inventors:

Christopher J. Power - Boulder CO
Ebrahim Firoozabady - Madison WI

Assignee:

Lubrizol Genetics, Inc. - Wickliffe OH

International Classification:

C12N 500

US Classification:

4352405

Abstract:

A method for regeneration of sunflower plants via shoot induction from cotyledon explants prepared from non-germinated sunflower embryos is described. Several methods for preparing explants by dissection of mature and immature fully-formed embryos are provided. Explants are prepared to insure that substantially all of the induced shoots derive from single cotyledon cells. Appropriately prepared explants are cultured on a shoot induction medium containing a combination of BA and NAA. Induced shoots are rooted and the resulting plantlets are grown into mature sunflower plants. This regeneration method is efficient, rapid and employs a convenient explant source, sunflower seed. This regeneration method is particularly useful in combination with methods for introduction of foreign DNA into sunflower cells and tissue.