Christopher W Silva

US Patent:

8445642, May 21, 2013

Filed:

Oct 14, 2008

Appl. No.:

12/251456

Inventors:

Bruce C. Onisko - Kensington CA, US
Christopher J. Silva - Albany CA, US
Jesus R. Requena - Briom, ES
John Mark Carter - Richmond CA, US

Assignee:

The United States of America as Represented by the Secretary of Agriculture - Washington DC

International Classification:

C07K 16/00
C07K 16/06
C07K 16/18

US Classification:

5303871, 5303881, 435 2, 4241841

Abstract:

The invention is directed to methods to distinguish among different protein conformers of the same protein such as proteins which form amyloid deposits. Using the methods of the invention, one or more protein conformers in a sample can be detected, differentiated, and quantitated. An example of a protein which is known to exist in at least two conformations is the normal prion protein (PrP) and its infectious isoform (PrP). The invention provides means to distinguish PrPfrom PrPand allows quantitation of each individually, even when the conformers are present together in a mixture. Thus, the methods of the invention can provide important tools for human and animal health.

US Patent:

20060110785, May 25, 2006

Filed:

Oct 13, 2005

Appl. No.:

11/249216

Inventors:

Bruce Onisko - Kensington CA, US
Christopher Silva - Albany CA, US
Jesus Requena - Briom, ES

Assignee:

The U.S.A. as rep. by the Secretary of Agriculture - Washington DC
Universidade De Santiago De Compostela - Santiago de Compostela

International Classification:

C12Q 1/37
G06F 19/00

US Classification:

435023000, 702019000

Abstract:

The invention is directed to methods to distinguish among different protein conformers of the same protein such as proteins which form amyloid deposits. Using the methods of the invention, one or more protein conformers in a sample can be detected, differentiated, and quantitated. An example of a protein which is known to exist in at least two conformations is the normal prion protein (PrP) and its infectious isoform (PrP). The invention provides means to distinguish PrPfrom PrPand allows quantitation of each individually, even when the conformers are present together in a mixture. Thus, the methods of the invention can provide important tools for human and animal health.