WellSpan Medical GroupWellSpan Behavioral Health 40 V Twin Dr STE 202, Gettysburg, PA 17325 (717)3392710 (phone), (717)3392711 (fax)
Languages:
English
Description:
Dr. Silva works in Gettysburg, PA and specializes in Psychologist. Dr. Silva is affiliated with Hanover Hospital and Wellspan Health Gettysburg Hospital.
Us Patents
Calibration Of Fluorescence Resonance Energy Transfer In Microscopy
Douglas C. Youvan - San Jose CA Christopher M. Silva - Sunnyvale CA Edward J. Bylina - San Jose CA William J. Coleman - Moutain View CA Michael R. Dilworth - Santa Cruz CA Mary M. Yang - San Jose CA
Assignee:
Kairos Scientific, Inc. - San Diego CA
International Classification:
G06K 900
US Classification:
382128, 382100, 382132, 382133
Abstract:
Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.
Solid Phase Enzyme Kinetics Screening In Microcolonies
Edward J. Bylina - San Jose CA William J. Coleman - Mountain View CA Michael R. Dilworth - Santa Cruz CA Christopher M. Silva - Sunnyvale CA Mary M. Yang - San Jose CA Douglas C. Youvan - San Jose CA
Assignee:
Kairos Scientific Inc. - Santa Clara CA
International Classification:
C12Q 144 C12Q 137 C12Q 154 C12Q 100
US Classification:
435 19
Abstract:
A MicroColonyImager instrument and solid phase methods to screen cells expressing mutagenized enzymes for enhanced activity. The MicroColonyImager instrument and methods permit high throughput screening of enzyme libraries by time course analyses of single-pixels, using either absorption, fluorescence or FRET. This high throughput assay can detect small differences in enzyme rates within microcolonies grown at a nearly confluent density on an assay disk. Each microcolony is analyzed simultaneously at single-pixel resolution, requiring less than 100 ml substrate/measurement. By simultaneously assaying different substrates tagged with spectrally distinct chromogenic or fluorogenic reporters, the substrate specificity of an enzyme can be changed.
Name / Title
Company / Classification
Phones & Addresses
Mr. Christopher Silva Manager
Emergency Air Conditioning & Heating Air conditioning & Heating Contractors - Residential
4045 S Buffalo Dr., Ste A101-606, Las Vegas, NV 89147 (702)4837795
Emergency Air Conditioning & Heating Heating & Air Conditioning/hvac
4045 S Buffalo Dr, Las Vegas, NV 89147 4045 S Buffalo Dr Ste A101-606, Las Vegas, NV 89147 (702)4837795
Christopher Silva Director, Director, Secretary
SoundCure Medical Devices · Mfg Electromedical Equipment · Nonclassifiable Establishments
560 S Winchester Blvd SUITE 500, San Jose, CA 95128 Attn: Melissa Severs 560 S Winchester Blvd STE 500, San Jose, CA 95128 33 Arch St, Boston, MA 02110
Christopher J. Silva
TRI-COUNTY CREMATION SOCIETY, LLC
Christopher J. Silva Manager
E-ENERGY.COM, LLC
Christopher A. Silva President
SUBURBAN GROUP, INC
* 93 N 25 St, San Jose, CA 95116 93 N 25 St, San Jose, CA 95116
Access Databases Programming Customer Service Budgets Microsoft Office Microsoft Excel SQL PowerPoint Microsoft Word Sales Logistics Team Building Outlook Inventory Management Leadership Contract Management Strategic Planning Project Planning Process Improvement
Addicted to Techology. Time Traveler. Step-Father. Video Game Junkie. Trusting the Stars. Giving to Humanity. Adventure. Ink. Blogger. Impatient. Paint. Over Analyzer. Explorer. Book Worm. Photosh...