Courtney M Prochnow

US Patent:

20090163422, Jun 25, 2009

Filed:

Dec 19, 2008

Appl. No.:

12/340526

Inventors:

Xiaojiang Chen - Los Angeles CA, US
Myron Goodman - La Canada CA, US
Courtney Prochnow - Culver City CA, US
Ronda Bransteitter - Culver City CA, US

International Classification:

G01N 33/68
G06G 7/58
G01N 33/53
C12Q 1/68
A61P 31/18
A61K 38/16
C12Q 1/02
G01N 33/573

US Classification:

514 12, 703 11, 436 86, 436501, 435 71, 435 29, 435 6

Abstract:

Three-dimensional structure of APOBEC-2 and other structure models of APOBEC proteins obtained by computer modeling that bear similarity with a root-mean-square deviation (RMSD) of 2.0 with the APOBEC-2 monomer, dimer or tetramer. Uses of the three-dimensional structure of APOBEC-2 and models of APOBEC proteins particularly for structure-based drug design of compounds, peptides or mutant APOBEC proteins designed to treat Hyper-IgM-2 Syndrome, B cell lymphomas and lentivirus infections, particularly the human immunodeficiency virus (HIV) infection. Methods for identifying a compound that binds to any fragment of an APOBEC protein. The method includes obtaining the three dimensional structure of the APOBEC-2 monomer, dimer or tetramer and identifying or designing one or more compounds that bind, mimic, enhance, disrupt, or compete with interactions of APOBEC family proteins with themselves, their nucleic acid substrates and other cellular or viral proteins based on the three dimensional structure of the APOBEC-2 protein.

US Patent:

20100081621, Apr 1, 2010

Filed:

Aug 14, 2009

Appl. No.:

12/583141

Inventors:

Lauren Holden - Los Angeles CA, US
Courtney Prochnow - Los Angeles CA, US
Y. Paul Chang - Los Angeles CA, US
Ronda Bransteitter - Culver City CA, US
Linda Chelico - Los Angeles CA, US
Udayaditya Sen - Los Angeles CA, US
Myron F. Goodman - Los Angeles CA, US
Xiaojiang S. Chen - Los Angeles CA, US

International Classification:

A61K 38/04
G01N 33/566
G06G 7/48
G01N 33/53
C12Q 1/68
A61K 31/7088

US Classification:

514 12, 436501, 703 11, 435 71, 435 6, 514 44 R

Abstract:

The structure, function and methods associated with proteins from the APOBEC family, which are involved in diverse biological functions, is disclosed. In one embodiment, the structure of APOBEC-3G (Apo3G) is disclosed. In another embodiment, a method of using APOBEC-3G (Apo3G) and/or Apo3G-CD2 to restrict the replication of Human Immunodeficiency Virus (HIV) and Hepatitis B virus (HBV) via cytidine deamination on ssDNA or RNA binding is disclosed. In yet another embodiment, the high-resolution crystal structure of an enzymatically active APOBEC protein, the C-terminal deaminase domain of Apo3G (Apo3G-CD2) is disclosed.