Cynthia R Edwards

US Patent:

6384208, May 7, 2002

Filed:

Jul 15, 1999

Appl. No.:

09/354947

Inventors:

Cynthia A. Edwards - Menlo Park CA
Charles R. Cantor - Boston MA
Beth M. Andrews - Maynard MA
Lisa M. Turin - Redwood City CA
Kirk E. Fry - Palo Alto CA

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

C07H 2104

US Classification:

536 241, 536 231

Abstract:

The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

US Patent:

6869765, Mar 22, 2005

Filed:

Nov 13, 2001

Appl. No.:

09/993346

Inventors:

Cynthia A. Edwards - Menlo Park CA, US
Charles R. Cantor - Del Mar CA, US
Beth M. Andrews - Maynard MA, US
Lisa M. Turin - Redwood City CA, US
Kirk E. Fry - Palo Alto CA, US

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

C12Q001/68

US Classification:

435 6, 435 4, 435 71

Abstract:

The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

US Patent:

7189506, Mar 13, 2007

Filed:

Mar 3, 2000

Appl. No.:

09/518297

Inventors:

Moon Young Lim - Redwood City CA, US
Cynthia A. Edwards - Morgan Hill CA, US
Kirk E. Fry - Palo Alto CA, US
Thomas W. Bruice - Carlsbad CA, US
Douglas B. Starr - Mountain View CA, US
Megan E. Laurance - San Francisco CA, US
Yan Kwok - Sunnyvale CA, US
Albert W. Tam - San Francisco CA, US

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 6, 435 721, 435 723, 435 691, 4352351, 436501, 536 231, 536 234, 536 235, 536 236, 536 237

Abstract:

The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.

US Patent:

20030049689, Mar 13, 2003

Filed:

Jun 12, 2002

Appl. No.:

10/170729

Inventors:

Cynthia Edwards - Redwood City CA, US
Volker Schellenberger - Palo Alto CA, US
Christopher Murray - Soquel CA, US
Yiyou Chen - San Jose CA, US
Anthony Day - San Francisco CA, US

International Classification:

G01N033/53
G01N033/567
G01N033/574
C12P021/02
C12N005/06

US Classification:

435/007100, 435/007200, 435/069100, 435/325000, 435/007230

Abstract:

The present invention provides methods and compositions relating to polypeptides engineered to bind a target and have at least two functional domains.

US Patent:

20060141456, Jun 29, 2006

Filed:

Jun 9, 2003

Appl. No.:

10/514516

Inventors:

Cynthia Edwards - Palo Alto CA, US
Judith Fox - Palo Alto CA, US
Christopher Murray - Soquel CA, US
Volker Schellenberger - Palo Alto CA, US
Robert Tressler - Palo Alto CA, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The present invention provides methods and compositions for milieu-dependent binding of a targeted agent to a target, for example, for the milieu-dependent binding of a diagnostic or therapeutic molecule to a diseased, injured or infected organ, tissue or cell.

US Patent:

57441316, Apr 28, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/476876

Inventors:

Cynthia A. Edwards - Menlo Park CA
Kirk E. Fry - Palo Alto CA
Charles R. Cantor - Boston MA
Beth M. Andrews - Maynard MA

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

A61K 3174
G01N 33566
G01N 33558

US Classification:

424 7808

Abstract:

The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i. e. , fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

US Patent:

57260141, Mar 10, 1998

Filed:

Sep 17, 1993

Appl. No.:

8/123936

Inventors:

Cynthia A. Edwards - Menlo Park CA
Charles R. Cantor - Boston MA
Beth M. Andrews - Watertown MA
Lisa M. Turin - Berkeley CA

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

C12Q 168
C12P 1934
G01N 33566

US Classification:

435 6

Abstract:

The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

US Patent:

57167800, Feb 10, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/484499

Inventors:

Cynthia A. Edwards - Menlo Park CA
Kirk E. Fry - Palo Alto CA
Charles R. Cantor - Boston MA
Beth M. Andrews - Watertown MA

Assignee:

Genelabs Technologies, Inc. - Redwood City CA

International Classification:

C12Q 168
G01N 33566

US Classification:

435 6

Abstract:

The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i. e. , fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.