Cynthia L Hendrickson

US Patent:

20120238738, Sep 20, 2012

Filed:

Jun 13, 2011

Appl. No.:

13/513726

Inventors:

Cynthia Hendrickson - Wenham MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

C07H 21/04
C12P 19/34

US Classification:

536 231, 435 9152, 435 912

Abstract:

Compositions are provided that include a synthetic oligonucleotide characterized by a double-stranded region, a single-stranded region, a forward primer site, a reverse primer site and one or more cleavage sites therebetween. Methods of use for these compositions include adaptors for the amplification of DNA fragments.

US Patent:

20120244525, Sep 27, 2012

Filed:

Jun 5, 2012

Appl. No.:

13/488997

Inventors:

Cynthia Hendrickson - Wenham MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

C07H 21/04
C12Q 1/68
C12P 19/34

US Classification:

435 61, 536 231, 435 9153, 435 9152

Abstract:

Compositions are provided that include a synthetic oligonucleotide characterized by a double-stranded region, a single-stranded region, a forward primer site, a reverse primer site and one or more cleavage sites therebetween. Methods of use for these compositions include adapters for the amplification of DNA fragments.

US Patent:

20090191553, Jul 30, 2009

Filed:

Oct 1, 2008

Appl. No.:

12/243925

Inventors:

Cynthia Hendrickson - Wenham MA, US

Assignee:

Applied Biosystems Inc. - Foster City CA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

In various embodiments, the present teachings provide sequencing methods which facilitate enhancing the efficiency of ligation and/or increasing sequencing reads. Various embodiments of the methods enable sequencing through template regions for which complementary labeled extension probes are unavailable or insufficient. In various embodiments, one or more rounds of ligation with unlabeled extension probes can be used in addition to a round of ligation with labeled extension probe. In various embodiments, for example, such methods can facilitate extension on template polynucleotides that do not bind labeled extension probe in the first round of ligation.