Cynthia P Paul

US Patent:

20090042207, Feb 12, 2009

Filed:

Aug 21, 2008

Appl. No.:

12/195724

Inventors:

David R. Engelke - Ann Arbor MI, US
Paul D. Good - Grass Lake MI, US
Cynthia P. Paul - Northville MI, US

International Classification:

C12Q 1/68
C12N 15/87

US Classification:

435 6, 435455

Abstract:

The present invention relates to compositions and methods for intracellular expression and delivery of siRNAs in mammalian cells. The siRNA is transcribed intracellularly as a double stranded RNA of about 18 to about 25 base pairs long from an expression cassette. Intracellular expression of siRNA is effective at reducing or eliminating expression of the targeted genes, and is applicable to reverse genetic analysis of genes and to genetic therapy, as for example to inhibiting expression of pathogenic genes and oncogenes.

US Patent:

20030148519, Aug 7, 2003

Filed:

Nov 14, 2002

Appl. No.:

10/294957

Inventors:

David Engelke - Ann Arbor MI, US
Paul Good - Grass Lake MI, US
Cynthia Paul - Northville MI, US

International Classification:

C12N015/85
C12N005/06

US Classification:

435/455000, 435/325000, 435/320100

Abstract:

The present invention relates to compositions and methods for intracellular expression and delivery of siRNAs in mammalian cells. The siRNA is transcribed intracellularly as a double stranded RNA of about 18 to about 25 base pairs long from an expression cassette. Intracellular expression of siRNA is effective at reducing or eliminating expression of the targeted genes, and is applicable to reverse genetic analysis of genes and to genetic therapy, as for example to inhibiting expression of pathogenic genes and oncogenes.