Dana Christian Andersen

US Patent:

6610516, Aug 26, 2003

Filed:

Nov 27, 2000

Appl. No.:

09/723545

Inventors:

Dana C. Andersen - Redwood City CA
Tiffany M. Bridges - Burlingame CA
Martin Gawlitzek - Foster City CA
Cynthia A. Hoy - Hillsborough CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12P 2104

US Classification:

435 701, 435 691, 4352523, 435358, 530395

Abstract:

A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding a glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt and prefers an octahedral coordination geometry, and/or (c) a plasma component. In this process, the occupancy of an N-linked glycosylation site occupied only in a fraction of a glycoprotein is enhanced. Such culturing is preferably carried out at a temperature of between about 30Â C. and 35Â C. and/or in the presence of up to about 2 mM of a butyrate salt and/or in the presence of a cell-cycle inhibitor.

US Patent:

6716602, Apr 6, 2004

Filed:

Nov 1, 2001

Appl. No.:

10/000655

Inventors:

Dana Andersen - Redwood City CA
John Joly - San Mateo CA
Bradley R. Snedecor - Portola Valley CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12P 2106

US Classification:

435 691, 435 694, 435 712, 4352528, 435243

Abstract:

The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant host cell regulated by an inducible system.

US Patent:

20030077739, Apr 24, 2003

Filed:

Aug 26, 2002

Appl. No.:

10/227694

Inventors:

Laura Simmons - Burlingame CA, US
Dana Andersen - Redwood City CA, US

Assignee:

GENENTECH, INC. - South San Francisco CA

International Classification:

C12P021/02
C12N001/21
C07H021/04
C12N015/74
C07K016/00

US Classification:

435/069100, 435/252330, 435/320100, 530/388150, 536/023530

Abstract:

The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.

US Patent:

20060246542, Nov 2, 2006

Filed:

Feb 9, 2006

Appl. No.:

11/351886

Inventors:

Laura Simmons - Burlingame CA, US
Dana Andersen - Redwood City CA, US

Assignee:

GENENTECH, INC. - SOUTH SAN FRANCISCO CA

International Classification:

C12P 21/06
C07H 21/04
C12N 1/21
C07K 16/42
C07K 16/28
C07K 16/22
C12N 15/74

US Classification:

435069100, 435252300, 435471000, 530388100, 530388220, 530387200, 530388250, 536023530

Abstract:

The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.

US Patent:

20070015244, Jan 18, 2007

Filed:

May 22, 2006

Appl. No.:

11/438484

Inventors:

Laura Simmons - Burlingame CA, US
Dana Andersen - Redwood City CA, US

Assignee:

GENENTECH, INC. - SOUTH SAN FRANCISCO CA

International Classification:

C12P 21/06
C07H 21/04
C12N 1/21
C07K 16/18

US Classification:

435069100, 435252300, 435320100, 530388100, 536023500

Abstract:

The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.

US Patent:

20100256336, Oct 7, 2010

Filed:

Sep 17, 2007

Appl. No.:

12/441775

Inventors:

Inn Huam Yuk - Berkeley CA, US
Bradley Richard Snedecor - Portola Valley CA, US
Dana Christian Andersen - Menlo Park CA, US

International Classification:

C07K 16/28
C12P 21/02
C12P 21/08

US Classification:

5303873, 435 711, 530350, 5303871

Abstract:

A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.

US Patent:

20110244517, Oct 6, 2011

Filed:

Feb 23, 2011

Appl. No.:

13/033521

Inventors:

Laura Simmons - Burlingame CA, US
Dana Andersen - Redwood City CA, US

International Classification:

C12P 21/00
C12N 1/21
C12N 15/63

US Classification:

435 696, 43525233, 4353201

Abstract:

The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.

US Patent:

6506598, Jan 14, 2003

Filed:

Apr 21, 2000

Appl. No.:

09/553924

Inventors:

Dana C. Andersen - Redwood City CA
Tiffany M. Bridges - Burlingame CA
Martin Gawlitzek - Foster City CA
Cynthia A. Hoy - Hillsborough CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12N 506

US Classification:

435359, 435 696, 435358, 4352523, 530395

Abstract:

A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding said glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt and prefers an octahedral coordination geometry, and/or (c) a plasma component. In this process, the occupancy of an N-linked glycosylation site occupied only in a fraction of a glycoprotein is enhanced. Such culturing is preferably carried out at a temperature of between about 30Â C. and 35Â C. and/or in the presence of up to about 2 mM of a butyrate salt and/or in the presence of a cell-cycle inhibitor.