Daniel K Kim

US Patent:

8399221, Mar 19, 2013

Filed:

Nov 4, 2008

Appl. No.:

12/291010

Inventors:

Daniel Y. Kim - Centreville VA, US
Yexun Wang - Ellicott City MD, US

Assignee:

SABiosciences Corporation - Frederick MD

International Classification:

C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 911, 435 912, 536 231, 536 245

Abstract:

Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse transcription reactions are described. Using these reaction conditions greater sensitivity and specificity of amplification of small RNAs including miRNAs is achieved.

US Patent:

20210324377, Oct 21, 2021

Filed:

Sep 19, 2019

Appl. No.:

17/276619

Inventors:

- Germantown MD, US
Eric Lader - Boyds MD, US
Niels Tolstrup - Kokkedal, DK
Joerg Krummheuer - Holte, DK
Daniel Y. Kim - Brunswick MD, US
Sascha Strauss - Hilden, DE

International Classification:

C12N 15/10
C12Q 1/6848

Abstract:

The present disclosure provides methods and kits for inhibiting cDNA synthesis of unwanted RNA species during reverse transcription. The methods and kits provided herein use blocking oligonucleotides such as those comprising locked nucleic acids (LNAs).

US Patent:

20210024920, Jan 28, 2021

Filed:

Mar 26, 2019

Appl. No.:

17/041724

Inventors:

- Germantown MD, US
Quan PENG - Clarksburg MD, US
Daniel KIM - Brunswick MD, US

International Classification:

C12N 15/10
C12Q 1/6806

Abstract:

The invention relates to integrated DNA and RNA library preparations and methods of making and uses thereof, wherein the DNA molecules are labelled with a tag identifying the molecule as being a DNA molecule and the RNA molecules are labelled with a tag identifying the molecule as being a RNA molecule. The methods do not require physical separation of DNA and RNA. The methods output two separate libraries from DNA and RNA, respectively, which helps flexible manipulation on downstream sequencing platforms. The application also claims the DNA library and the cDNA library produced by the method and the DNA tag and the RNA tag used in the method.

US Patent:

20190132351, May 2, 2019

Filed:

Dec 26, 2018

Appl. No.:

16/232816

Inventors:

- Houston TX, US
Daniel Jonghoon KIM - Fairfax VA, US
Erik WELLS - Holly Springs NC, US

International Classification:

H04L 29/06
G06F 16/23
H04L 12/24

Abstract:

A method and apparatus useful for data risk monitoring and management includes configuration and analysis of data flows to identify and assess risk and compliance to various regulatory standards and business practices. The evaluation of monitored data flows are then further used to identify potential security risks based on deviation from expected flows or compliant handling methods.

US Patent:

20170032129, Feb 2, 2017

Filed:

Aug 1, 2016

Appl. No.:

15/225289

Inventors:

- Houston TX, US
Daniel Jonghoon Kim - Fairfax VA, US

International Classification:

G06F 21/57

Abstract:

A method and apparatus useful for data risk monitoring and management includes configuration and analysis of data flows to identify and assess risk and compliance to various regulatory standards and business practices. The evaluation of monitored data flows are then further used to identify potential security risks based on deviation from expected flows or compliant handling methods.