Daniel J Wagner

US Patent:

50454781, Sep 3, 1991

Filed:

Oct 9, 1987

Appl. No.:

7/106385

Inventors:

Daniel B. Wagner - Raleigh NC
Uri Piran - Norwood MA

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33532

US Classification:

436501

Abstract:

Sacs comprising a compound having a hydrophilic peptide radical are prepared and are believed to have improved stability as a result of hydrogen bonding. The sacs, including a detectable marker and derivatized with a ligand may be used as a tracer in an assay. At least a portion of the sac is formed from a compound having the following structural formula: X-Y-Z, wherein X is a hydrophobic radical; Y is a hydrophilic peptide; and Z is a radical which includes a non-hydrolyzable polar group.

US Patent:

50010549, Mar 19, 1991

Filed:

Jul 31, 1990

Appl. No.:

7/560913

Inventors:

Daniel B. Wagner - Raleigh NC

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12M 134

US Classification:

435 14

Abstract:

A method for monitoring the glucose level in a body fluid uses an apparatus which includes a conjugate of glucose oxidase and a fluorescent dye coated onto an optical fiber in contact with the body fluid, a source of excitation light and a fluorescence emission detector. Glucose is oxidized by oxygen in the body fluid causing a decrease in oxygen concentration at the enzyme. The fluorescent dye is sensitive to oxygen quenching so that, when the oxygen concentration decreases, fluorescence emission increases in direct proportion to the glucose concentration in the fluid.

US Patent:

46668306, May 19, 1987

Filed:

Aug 23, 1984

Appl. No.:

6/643478

Inventors:

Daniel B. Wagner - Raleigh NC

International Classification:

G01N 3353

US Classification:

435 7

Abstract:

In an assay for an analyte, the tracer is comprised of a sac lysing agent, which lyses sacs containing detectable marker and sac lysing agent in inactive form which is activated when released from the sacs to provide a cascade effect which increases sensitivity.

US Patent:

47176768, Jan 5, 1988

Filed:

Mar 3, 1986

Appl. No.:

6/835781

Inventors:

Daniel B. Wagner - Raleigh NC
Uri Piran - Norwood MA

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33544

US Classification:

436501

Abstract:

Sacs comprising a compound having a hydrophilic peptide radical are prepared and are believed to have improved stability as a result of hydrogen bonding. The sacs, including a detectable marker and derivatized with a ligand may be used as a tracer in an assay.

US Patent:

43105041, Jan 12, 1982

Filed:

Sep 12, 1979

Appl. No.:

6/075060

Inventors:

Sara Derfler - Jerusalem, IL
Yehuday Tamir - Jerusalem, IL
Daniel B. Wagner - Raleigh NC

Assignee:

Ames-Yissum Ltd. - Jerusalem

International Classification:

G01N 3348
G01T 100
B65D 7100

US Classification:

424 1

Abstract:

A dry radioimmunoassay test composition comprising a mixture of a radiolabelled form of a ligand, i. e. , a hapten or an antigen, an antibody for the ligand, and a heavy metal cation, e. g. , cupric ion, to inhibit the complex-forming reaction between the radiolabelled ligand and the antibody. A method for preparing the dry test composition is also provided. In use in a radioimmunoassay, the dry test composition is reconstituted with an aqueous liquid and combined with a test sample and a chelating agent to bind the heavy metal cation, permitting reaction among any ligand present in the sample, the radiolabelled ligand, and the antibody. A test kit for performing such radioimmunoassay is also provided.

US Patent:

46955542, Sep 22, 1987

Filed:

Mar 27, 1985

Appl. No.:

6/716533

Inventors:

James P. O'Connell - Chapel Hill NC
Uri Piran - Norwood MA
Daniel B. Wagner - Raleigh NC

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33533
B01J 1302

US Classification:

436528

Abstract:

A sac, in particular a vesicle, including as a detectable marker an absorbing dye having an extinction coefficient of at least 100,000 and a solubility in water of at least 0. 1M; in particular, sulforhodamine B or salt thereof. The sacs have improved stability to leakage and are useful in a variety of assays; in particular, when sensitized with a ligand.

US Patent:

50174738, May 21, 1991

Filed:

May 26, 1987

Appl. No.:

7/054466

Inventors:

Daniel B. Wagner - Raleigh NC

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 3353
G01N 2176
G01N 33543
C12Q 128

US Classification:

435 792

Abstract:

A method for separation-free solid phase immunoassay of an analyte includes contacting an antianalyte attached to the surface of a solid support with the analyte, a light absorbing material and a tracer for the analyte which includes a label. The resulting mixture is incubated and chemiluminescence is generated. All of the chemiluminescence is absorbed by the light absorbing material except that associated with the bound tracer whereby the only emission detected is due to the bound tracer. Since emission from free tracer in the fluid phase of the assay medium is not detected, separation of the bound and free fractions is unnecessary. The invention includes a kit of materials useful in performing an immunoassay in accordance with the method of the invention.

US Patent:

49406698, Jul 10, 1990

Filed:

May 15, 1987

Appl. No.:

7/049971

Inventors:

Daniel B. Wagner - Raleigh NC
Glenn P. Vonk - Fuquay-Varina NC

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33566
G01N 33543
G01N 33544
G01N 33532

US Classification:

436501

Abstract:

A sac, in particular a vesicle, having a detectable metal, in particular a rear earth metal, encapsulated therein. The sac may be sensitized with a ligand and used in an assay. The vesicle is stored and/or used in an aqueous solution which includes the rare earth metal to increase the concentration of metal in the sac.