David A Comstock

US Patent:

20110201507, Aug 18, 2011

Filed:

Dec 1, 2010

Appl. No.:

12/958353

Inventors:

Richard P. Rava - Redwood City CA, US
Manjula Chinnappa - Foster City CA, US
David A. Comstock - Sunnyvale CA, US
Gabrielle Heilek - Mountain View CA, US
Brian Kent Rhees - Chandler AZ, US

International Classification:

C40B 30/00
C40B 60/08
G06F 19/00

US Classification:

506 7, 506 37, 702 20

Abstract:

The invention provides methods for determining aneuploidy and/or fetal fraction in maternal samples comprising fetal and maternal cfDNA by massively parallel sequencing. The method comprises a novel protocol for preparing sequencing libraries that unexpectedly improves the quality of library DNA while expediting the process of analysis of samples for prenatal diagnoses.

US Patent:

20110224087, Sep 15, 2011

Filed:

Dec 1, 2010

Appl. No.:

12/958356

Inventors:

Stephen Quake - Stanford CA, US
Richard P. Rava - Redwood City CA, US
Manjula Chinnappa - Foster City CA, US
David A. Comstock - Sunnyvale CA, US
Gabrielle Heilek - Mountain View CA, US

International Classification:

C40B 30/00
C40B 40/06

US Classification:

506 7, 506 16

Abstract:

The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.

US Patent:

20120010085, Jan 12, 2012

Filed:

Dec 1, 2010

Appl. No.:

12/958347

Inventors:

Richard P. RAVA - Redwood City CA, US
Manjula CHINNAPPA - Foster City CA, US
David A. COMSTOCK - Sunnyvale CA, US
Gabrielle HEILEK - Mountain View CA, US
Michael HUNKAPILLER - San Carlos CA, US

International Classification:

C40B 20/00
C07H 21/00
C40B 30/00

US Classification:

506 2, 506 7, 536 2433

Abstract:

The invention provides compositions and methods for determining the fraction of fetal nucleic acids in a maternal sample comprising a mixture of fetal and maternal nucleic acids. The fraction of fetal nucleic acids can be used in determining the presence or absence of fetal aneuploidy.

US Patent:

20120100548, Apr 26, 2012

Filed:

Jul 26, 2011

Appl. No.:

13/191366

Inventors:

Richard P. RAVA - Redwood City CA, US
David A. COMSTOCK - Sunnyvale CA, US
Brian K. RHEES - Chandler AZ, US

Assignee:

Verinata Health, Inc. - San Carlos CA

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

The invention provides a method for determining copy number variations (CNV) of a sequence of interest in a test sample that comprises a mixture of nucleic acids that are known or are suspected to differ in the amount of one or more sequence of interest. The method comprises a statistical approach that accounts for accrued variability stemming from process-related, interchromosomal and inter-sequencing variability. The method is applicable to determining CNV of any fetal aneuploidy, and CNVs known or suspected to be associated with a variety of medical conditions. CNV that can be determined according to the present method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions and/or duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample. Any aneuploidy can be determined from sequencing information that is obtained by sequencing only once the nucleic acids of a test sample.

US Patent:

20120149582, Jun 14, 2012

Filed:

Feb 2, 2012

Appl. No.:

13/364809

Inventors:

Richard P. Rava - Redwood City CA, US
David A. Comstock - Sunnyvale CA, US
Brian K. Rhees - Chandler AZ, US

Assignee:

Verinata Health, Inc. - Redwood City CA

International Classification:

C40B 20/00

US Classification:

506 2

Abstract:

The invention provides a method for determining copy number variations (CNV) of a sequence of interest in a test sample that comprises a mixture of nucleic acids that are known or are suspected to differ in the amount of one or more sequence of interest. The method comprises a statistical approach that accounts for accrued variability stemming from process-related, interchromosomal and inter-sequencing variability. The method is applicable to determining CNV of any fetal aneuploidy, and CNVs known or suspected to be associated with a variety of medical conditions. CNV that can be determined according to the present method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions and/or duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample.

US Patent:

20120149583, Jun 14, 2012

Filed:

Feb 2, 2012

Appl. No.:

13/365134

Inventors:

Richard P. RAVA - Redwood City CA, US
David A. Comstock - Sunnyvale CA, US
Brian K. Rhees - Chandler AZ, US

Assignee:

Verinata Health, Inc. - Redwood City CA

International Classification:

C40B 20/00

US Classification:

506 2

Abstract:

The invention provides a method for determining copy number variations (CNV) of a sequence of interest in a test sample that comprises a mixture of nucleic acids that are known or are suspected to differ in the amount of one or more sequence of interest. The method comprises a statistical approach that accounts for accrued variability stemming from process-related, interchromosomal and inter-sequencing variability. The method is applicable to determining CNV of any fetal aneuploidy, and CNVs known or suspected to be associated with a variety of medical conditions. CNV that can be determined according to the present method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions and/or duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample.

US Patent:

20120165203, Jun 28, 2012

Filed:

Feb 2, 2012

Appl. No.:

13/365240

Inventors:

Stephen Quake - Stanford CA, US
Richard P. Rava - Redwood City CA, US
Manjula Chinnappa - Foster City CA, US
David A. Comstock - Sunnyvale CA, US
Gabrielle Heilek - Mountain View CA, US

Assignee:

Verinata Health, Inc. - Redwood City CA

International Classification:

C12Q 1/68
C40B 20/00

US Classification:

506 2, 435 611

Abstract:

The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.

US Patent:

20120214678, Aug 23, 2012

Filed:

May 1, 2012

Appl. No.:

13/461582

Inventors:

RICHARD P. RAVA - REDWOOD CITY CA, US
MANJULA CHINNAPPA - FOSTER CITY CA, US
DAVID A. COMSTOCK - SAN FRANCISCO CA, US
GABRIELLE HEILEK - MOUNTAIN VIEW CA, US
MICHAEL HUNKAPILLER - SAN CARLOS CA, US

Assignee:

VERINATA HEALTH, INC. - REDWOOD CITY CA

International Classification:

C40B 20/00

US Classification:

506 2

Abstract:

The invention provides compositions and methods for determining the fraction of fetal nucleic acids in a maternal sample comprising a mixture of fetal and maternal nucleic acids. The fraction of fetal nucleic acids can be used in determining the presence or absence of fetal aneuploidy.