David R Graves

US Patent:

20030099930, May 29, 2003

Filed:

Oct 29, 2001

Appl. No.:

10/052972

Inventors:

David Graves - Devon PA, US
David Cossaboon - Germantown MD, US
Richard Su - Arcadia CA, US
Paolo Fortina - Philadelphia PA, US
Saul Surrey - Chadds Ford PA, US
Steve McKenzie - Springfield PA, US

International Classification:

C12Q001/70
C12Q001/68
G01N033/53
G01N033/567
C12M001/34
B05D003/00

US Classification:

435/005000, 435/006000, 435/007200, 427/002110, 435/287200

Abstract:

Disclosed are nucleic acid microarray devices and their method of preparation employing dendrimeric polyamines to bind nucleic acid probes to solid support surfaces thereby enhancing the surface molecule density and hybridization efficiency of nucleic acids in the microarray. Also disclosed are methods of using the resulting microarray device for analyzing test samples containing target molecules.

US Patent:

20030119028, Jun 26, 2003

Filed:

Aug 7, 2002

Appl. No.:

10/213770

Inventors:

David Graves - Devon PA, US
Paolo Fortina - Philadelphia PA, US
Richard Su - Arcadia CA, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

A device and methods are provided for enhancing DNA microarray hybridization speed and discrimination efficiency by means of an electric field.

US Patent:

20030124659, Jul 3, 2003

Filed:

Jun 7, 2002

Appl. No.:

10/165186

Inventors:

David Graves - Devon PA, US

International Classification:

C12P021/06

US Classification:

435/069100

Abstract:

A “process of elimination” method of creating mixtures of labeled particles, allowing the particles to interact, documenting particle interactions or lack thereof, and collating information derived from more than one experiment, with each experiment combining particles from one or more mixtures with other particles.

US Patent:

20070111204, May 17, 2007

Filed:

Jun 25, 2004

Appl. No.:

10/562415

Inventors:

Kathleen Delgrosso - Aldan PA, US
Paolo Fortina - Philadelphia PA, US
David Graves - Devon PA, US
Saul Surrey - Chadds Ford PA, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536025320

Abstract:

The invention provides methods and diagnostic test kits for detecting target nucleic acid sequence variations in a sample. In particular, a plurality of oligonucleotide probe sets is provided. Each set has a target specific portion and a barcode. The target specific portions of the probes are suitable for ligation together when hybridized adjacent to one another on a corresponding target polynucleotide. The ligated product contains a barcode that binds to a probe attached to a substrate and hence facilitate the capture of the ligated product on the solid support. Detection is carried out with a gold nanoparticle-attached barcode that hybridizes with the barcode on the ligated product.

US Patent:

46751130, Jun 23, 1987

Filed:

Sep 12, 1986

Appl. No.:

6/906475

Inventors:

David J. Graves - Devon PA
Mark A. Burns - Fairfield NJ

Assignee:

University Patents, Inc. - Westport CT

International Classification:

B01D 1508

US Classification:

210635

Abstract:

Disclosed is a method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.

US Patent:

51678110, Dec 1, 1992

Filed:

Mar 4, 1992

Appl. No.:

7/845346

Inventors:

David J Graves - Devon PA
Mark A. Burns - Fairfield NJ

Assignee:

Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

B01D 1508

US Classification:

2101982

Abstract:

Disclosed is a method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.

US Patent:

51678128, Dec 1, 1992

Filed:

Jan 17, 1992

Appl. No.:

7/824751

Inventors:

David J. Graves - Devon PA
Mark A. Burns - Fairfield NJ

Assignee:

Trustees of the Univ. of Penna. - Philadelphia PA

International Classification:

B01D 1508

US Classification:

2101982

Abstract:

Disclosed is a method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.

US Patent:

50551949, Oct 8, 1991

Filed:

Jul 28, 1989

Appl. No.:

7/387073

Inventors:

Victor Goetz - Philadelphia PA
David J. Graves - Devon PA

Assignee:

University of Pennsylvania - Philadelphia PA

International Classification:

B01D 1508

US Classification:

210635

Abstract:

A method of, and an apparatus for, conducting chromatographic separations of multi-component systems utilizing silica-coated, magnetically susceptible particles in an MSFB is disclosed. A support material comprised of beads having a magnetic core surrounded by a silica derivative porous coating is used.