David J Schofield

US Patent:

20190100811, Apr 4, 2019

Filed:

Oct 1, 2018

Appl. No.:

16/149019

Inventors:

- San Diego CA, US
- Dublin OH, US
Todd Denison PACK - San Diego CA, US
David A. SCHOFIELD - Hollywood SC, US

International Classification:

C12Q 1/6897
C12Q 1/04

Abstract:

Methods for determining bacterial identity and susceptibility or resistance to antibiotic or antimicrobial agents are provided. In one embodiment, the bacteria is cultured in the presence or absence or the antibiotic agent to generate a plurality of primary cultures, which are then cultured in the presence or absence of transforming phages to generate a first secondary culture that comprise transformed bacteria that have been treated with the antibiotic agent and a second secondary culture that comprises transformed bacteria that have not been treated with the antibiotic agent. The recombinant phages are specific to the bacteria and comprise a heterologous marker. The susceptibility or resistance of the bacteria to the antibiotic or antimicrobial agent is determined by comparing a level or activity of the marker in the first and second secondary cultures.