David A Stillman

US Patent:

8097431, Jan 17, 2012

Filed:

Aug 4, 2005

Appl. No.:

11/631725

Inventors:

A. Wayne Meikle - Salt Lake City UT, US
David J. Stillman - Salt Lake City UT, US
Jared M. Orrock - Salt Lake City UT, US
Alan H. Terry - Bountiful UT, US
Tanya M. Sandrock - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

G01N 33/53
C12Q 1/66
C12Q 1/48
C12Q 1/42
C07H 21/04
C12P 21/06
C12N 15/00
C12N 5/00

US Classification:

435 78, 435 8, 435 15, 435 21, 435 691, 4353201, 435325, 435810, 536 234, 536 235

Abstract:

The present invention provides for methods and systems for detecting steroids. Examples of such steroids include estrogen, progesterone, androgen, testosterone, and derivatives and analogs thereof. Systems useful for carrying out the method include tripartite constructs including a DNA-binding domain, a ligand binding domain, and an activation domain. The present invention provides numerous improvements over previous diagnostic systems for detection of steroids, such advantages include that the method allows for detection of steroid analogs and derivatives, whose structures may not yet be known, the method is generally applicable to a wide variety of organisms, and numerous ligand binding domains may be used in conjunction with the present system.