David K Sykes

US Patent:

6884589, Apr 26, 2005

Filed:

Nov 30, 2001

Appl. No.:

10/432057

Inventors:

Mark P. Kamps - Carlsbad CA, US
David B. Sykes - La Jolla CA, US

Assignee:

Regents of the University of California - Oakland CA

International Classification:

C12Q001/68

US Classification:

435 6, 435325, 530350, 536 234

Abstract:

The invention is a genetic construct comprising the coding sequence for a hormone dependent E2a-Pbx1 protein. The invention is the use of the construct to generate hematopoietic cell lines arrested in differentiation upon exposure to the appropriate hormone. Upon removal of the hormone, cells undergo normal, synchronous differentiation providing a system for the study of normal differentiation. Expression of a number of oncogenes in the cells maintains the cells in an undifferentiated state, providing a model for the study the mechanisms of leukemia, and for the testing of pharmacological agents for the treatment and amelioration of the disease.

US Patent:

20090068157, Mar 12, 2009

Filed:

Dec 9, 2005

Appl. No.:

11/792988

Inventors:

Mark Kamps - Carlsbad CA, US
David Sykes - Boston MA, US

International Classification:

A61K 35/12
C12N 5/06
C12N 15/87
C12N 5/08
C12Q 1/68
A01K 67/027
A61P 29/00
C12Q 1/02
C12N 15/11
C12N 15/00
C07K 14/00

US Classification:

424 9321, 435377, 435455, 435456, 435366, 435352, 435354, 435 6, 800 3, 435 29, 536 234, 4353201, 530350

Abstract:

A method to generate unlimited numbers of macrophage/dendritic cells or neutrophils from mice, using conditional Hox oncoproteins is disclosed. The invention further includes the establishment of a system to investigate immune responses to microorganisms or diseases involving chronic inflammation.