David L Wolf

US Patent:

6485963, Nov 26, 2002

Filed:

Jun 2, 2000

Appl. No.:

09/587028

Inventors:

David A. Wolf - Houston TX
Thomas J. Goodwin - Friendswood TX

Assignee:

The United States of America as represented by the Administrator of the National Aeronautics and Space Administration - Washington DC

International Classification:

C12M 110

US Classification:

4352982, 4352991

Abstract:

The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

US Patent:

6673597, Jan 6, 2004

Filed:

Feb 28, 2001

Appl. No.:

09/798854

Inventors:

David A. Wolf - Houston TX
Thomas J. Goodwin - Friendswood TX

Assignee:

The United States of America as represented by the Administrator of the National Aeronautics and Space Administration - Washington DC

International Classification:

C12M 110

US Classification:

4352982, 4352991

Abstract:

The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

US Patent:

20070190520, Aug 16, 2007

Filed:

Jan 31, 2007

Appl. No.:

11/669637

Inventors:

David WOLF - Houston TX, US
Donnie RUDD - Sugar Land TX, US

International Classification:

C12Q 1/70
C12Q 1/68
C12Q 1/00

US Classification:

435004000, 435005000, 435006000, 977902000

Abstract:

A method of characterizing a biologically active compound by placing a cell mixture into a rotatable bioreactor to initiate a three-dimensional culture comprising a biological component and at least one cell, controllably expanding the cells in the rotatable bioreactor and testing the biological component to characterize the biologically active compound. The present invention may also preferably comprise exposing the cells to a time varying electromagnetic force.

US Patent:

20080075700, Mar 27, 2008

Filed:

Aug 20, 2007

Appl. No.:

11/894151

Inventors:

David Wolf - Houston TX, US
Donnie Rudd - Sugar Land TX, US

International Classification:

A61K 35/00
A61P 7/12
C12N 5/06

US Classification:

424093700, 435372000

Abstract:

The present invention is directed to the expansion, preferably TVEMF-expansion, of mammalian blood stem cells in a rotating bioreactor, preferably a TVEMF-bioreactor, to compositions resulting from the expanded and TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions.

US Patent:

20080075704, Mar 27, 2008

Filed:

Aug 20, 2007

Appl. No.:

11/894098

Inventors:

David Wolf - Houston TX, US
Donnie Rudd - Sugar Land TX, US

International Classification:

A01N 1/02
A61K 35/00
A61P 43/00
C12N 13/00
C12N 15/01
C12N 5/06
C12Q 1/02

US Classification:

424093210, 435173100, 435002000, 435029000, 435372000, 435446000

Abstract:

The present invention is directed to the process of preparing an expanded peripheral blood stem cell composition, preparing a TVEMF-expanded peripheral blood stem cell composition, methods of producing the same, and to repair of tissue and/or function with expanded, and TVEMF-expanded, peripheral blood stem cells.

US Patent:

20080097530, Apr 24, 2008

Filed:

Oct 18, 2007

Appl. No.:

11/874410

Inventors:

Philip MUCCIO - Columbus OH, US
Robert DENNIS - Chapel Hill NC, US
David WOLF - Houston TX, US
Marc LABBE - Houston TX, US

International Classification:

A61N 1/34
A61N 1/00
A61N 1/36

US Classification:

607003000, 607046000, 607050000

Abstract:

A system for stimulating tissue to relieve pain and repair and/or regenerate tissue comprising a garment that has an electrode, a programmable electrical stimulation device, a time varying electromagnetic field generator, and a source that provides an electrical current. Also provided is a method wherein the electrode provides the user with a stimulating current and the time varying electromagnetic field provides the user with a time varying electromagnetic field either simultaneously, alternating, and/or sequentially.

US Patent:

20080138415, Jun 12, 2008

Filed:

Sep 24, 2007

Appl. No.:

11/859952

Inventors:

Mehboob HUSSAIN - Baltimore MD, US
Robert G. DENNIS - Raleigh NC, US
David A. WOLF - Houston TX, US

International Classification:

A61K 35/12
A61K 9/14
C12N 5/06
C12N 13/00
A61P 43/00

US Classification:

424486, 424 937, 435377, 435366, 4351731

Abstract:

A cellular composition comprising a mesenchymal stem cell product and a biogel with methods for preparing and for using the composition. Tissue constructs made with the composition are also disclosed.

US Patent:

20090068153, Mar 12, 2009

Filed:

Sep 6, 2007

Appl. No.:

11/850775

Inventors:

Francesca P. Vitelli - Houston TX, US
David A. Wolf - Houston TX, US
Donnie Rudd - Sugar Land TX, US

International Classification:

C12N 5/08
A61K 35/50

US Classification:

424 937, 435366

Abstract:

A method of extracting human progenitor cells from perivascular tissue of human umbilical cord. The extracted cells are then co-cultured with hematopoetic stem cells and are useful to grow and repair human tissues including bone. Also included are related methods and compositions related thereto.