David J Wolf

US Patent:

D454483, Mar 19, 2002

Filed:

Nov 30, 2000

Appl. No.:

29/133491

Inventors:

David Wolf - Newton MA

Assignee:

Allied Bolts, Inc. - Hollis NY

International Classification:

0808

US Classification:

D 8394

US Patent:

6844166, Jan 18, 2005

Filed:

Sep 10, 1999

Appl. No.:

09/394265

Inventors:

David E. Wolf - Hudson MA, US

Assignee:

Sensor Technologies Inc. - Shrewsbury MA

International Classification:

G01N 33537

US Classification:

435 793, 435 71, 435 792, 435 14, 436501, 436 14, 436147, 436164, 436172, 436 95, 436531, 436545, 436546, 436805, 436807, 436815, 436827

Abstract:

The invention is based on the discovery of reduced valency carbohydrate binding ligands (CBLs) that can be used to to detect or quantitate (i. e. , evaluate) carbohydrates in a sample. CBLs can be used with fluorescence resonance energy transfer (FRET) to evaluate free carbohydrates or those within a carbohydrate containing compound.

US Patent:

7520808, Apr 21, 2009

Filed:

Jun 15, 2004

Appl. No.:

10/868324

Inventors:

David Wolf - Newton MA, US

International Classification:

G06F 17/00

US Classification:

463 17

Abstract:

An online lottery game having periodic drawings of a series of digits. Money derived from the sale of lottery tickets is allocated into a plurality of winning pools in which the winning pool consisting of all of the numbers is smaller than at least one and preferably all of the other winning pools consisting of less than all of the numbers. Money allocated to pools are carried forward to successive drawings in the event there are no winners of that particular pool.

US Patent:

8075382, Dec 13, 2011

Filed:

Feb 26, 2009

Appl. No.:

12/393396

Inventors:

David Wolf - Newton MA, US

International Classification:

G06F 17/00

US Classification:

463 16

Abstract:

An online lottery game having periodic drawings of a series of digits. Money derived from the sale of lottery tickets is allocated into a plurality of winning pools in which the winning pool consisting of all of the numbers is smaller than at least one and preferably all of the other winning pools consisting of less than all of the numbers. Money allocated to pools are carried forward to successive drawings in the event there are no winners of that particular pool.

US Patent:

7268878, Sep 11, 2007

Filed:

Aug 1, 2003

Appl. No.:

10/633385

Inventors:

David E. Wolf - Sudbury MA, US
Dylan A. Bulseco - Princeton MA, US

Assignee:

Sensor Technologies LLC - Shrewsbury MA

International Classification:

G01N 21/25

US Classification:

356417

Abstract:

Disclosed is a portable fluorescence correlation spectroscopy instrument that includes an excitation source, at least one of a light focusing element positioned to receive light emitted by the excitation source, a detector for detecting light, the detector positioned to receive light emitted by a sample excited by the excitation source, and a correlator coupled to the detector, the correlator for processing data received at the detector and providing data including autocorrelation data, crosscorrelation data, or a combination thereof.

US Patent:

20030087311, May 8, 2003

Filed:

Nov 7, 2002

Appl. No.:

10/290971

Inventors:

David Wolf - Sudbury MA, US

International Classification:

C12Q001/68
G01N033/53
C12M001/34
G01N033/543

US Classification:

435/007100, 436/518000, 435/006000, 435/287200

Abstract:

A method of identifying an analyte-ligand binding pair that exhibits non-radiative fluorescence resonance energy transfer (FRET) using a combinatorial library. The method includes a) obtaining an analyte binding ligand from a combinatorial library that includes ligands, and b) attaching a label at least one of the analyte binding ligand and an analyte-analogue with at least one of a first component and a second component of a non-radiative fluorescence resonance energy transfer donor-acceptor pair (FRET pair) such that FRET occurs when the analyte-analogue is bound to the analyte binding ligand, and a change in FRET occurs when the analyte-analogue is not bound to the analyte binding ligand. The method also includes contacting a combinatorial library of ligands, which are optionally labeled with a component of a FRET pair, with analyte-analogue, which is optionally labeled with a component of a FRET pair, and detecting the presence of FRET.

US Patent:

20030170278, Sep 11, 2003

Filed:

Mar 11, 2002

Appl. No.:

10/095503

Inventors:

David Wolf - Sudbury MA, US
Claire McGrath - West Boylston MA, US
Willem Kuhtreiber - Shrewsbury MA, US
Jeanine Lorusso - Sterling MA, US
Angela Pollard - Westminster MA, US

International Classification:

A61K009/00
A61K009/48
A61K009/50

US Classification:

424/400000, 424/451000, 424/093700

Abstract:

A method of determining a suitable microreactor for a predetermined host including identifying a suitable microreactor diameter, optionally identifying a minimum second matrix thickness, and optionally identifying a microreactor having a centrally located microcapsule. A microreactor having a diameter such that, when implanted in a higher order mammal, the microreactor exhibits no greater than 25% fibrosis for at least 14 days after implantation. A microreactor that includes a semipermeable membrane capable of eliciting a fibrotic response from a higher order mammal, and a second matrix surrounding the semipermeable membrane, the second matrix being of a thickness sufficient to prevent nucleation of fibrosis by the semipermeable membrane when the microreactor is implanted in a higher order mammal for at least 14 days.

US Patent:

20040082080, Apr 29, 2004

Filed:

Aug 1, 2003

Appl. No.:

10/632725

Inventors:

David Wolf - Sudbury MA, US
Dylan Bulseco - Princeton MA, US

International Classification:

C12Q001/68
G01N033/53
G01N033/543

US Classification:

436/518000, 435/006000, 435/007100

Abstract:

The invention features a method of assaying for the interaction of a probe and an unknown target, said method including a) exciting a sample with radiation, the sample including at least one unknown target, at least one probe, and at least one fluorescent tag, b) measuring the fluorescence from a subvolume of the sample, and c) analyzing the fluctuations of the fluorescence.