Dean S Burgi

US Patent:

20050115837, Jun 2, 2005

Filed:

Dec 1, 2003

Appl. No.:

10/726321

Inventors:

Dean Burgi - Sunnyvale CA, US
Stephen Williams - San Carlos CA, US

International Classification:

G01N027/453

US Classification:

204452000, 204603000

Abstract:

The present invention is directed to methods for identifying one or more analytes in a sample using electrophoresis. In one embodiment, the method comprises performing an electrophoretic separation by applying a potential across the separation path and thus generating a current and power therein and producing an electropherogram, integrating the current or the power to determine the cumulative current or power as a function of the separation time, transforming the electropherogram to a second electropherogram representing the signal as a function of the cumulative current or power, and identifying in the second electropherogram peaks that are correlated with the analytes in the sample. The invention also provides systems for performing the analysis and identification methods, as well as computer-readable products for performing the steps associated with the above methods.

US Patent:

20050161329, Jul 28, 2005

Filed:

Apr 14, 2003

Appl. No.:

10/511282

Inventors:

Katariina Hutterer - Santa Barbara CA, US
Nels Olson - La Jolla CA, US
Stevan Jovanovich - Livermore CA, US
Thomas Armstrong - Santa Clara CA, US
Dean Burgi - Sunnyvale CA, US
Vladislav Dolnik - Mountain View CA, US

International Classification:

G01N027/453

US Classification:

204456000, 204605000

Abstract:

Multi-capillary systems for high-throughput electrophoretic separation and detection of biomolecules are disclosed. One embodiment of the invention uses galactomannans as a size-sieving matrix for multi-channel electrophoretic separations of biomolecules. Multi-color detection for the simultaneous analysis of controls and standards in the same channels as the samples, and endogenous fluorescence detection are also disclosed. Another embodiment of the invention is a two dimensional system for separation of complex samples, using multiplexed capillary electrophoresis system as the second dimension, with a fraction collection step connecting the two separation steps. The systems allow for separations to be accomplished with a highly parallel manner, or in a two-dimensional format.

US Patent:

20070158193, Jul 12, 2007

Filed:

Aug 7, 2006

Appl. No.:

11/462990

Inventors:

Dean Burgi - South San Francisco CA, US
Stephen Williams - South San Francisco CA, US

International Classification:

B01D 57/02
G01N 27/00

US Classification:

204452000, 204603000

Abstract:

The present invention is directed to methods for identifying one or more analytes in a sample using electrophoresis. In one embodiment, the method comprises performing an electrophoretic separation by applying a potential across the separation path and thus generating a current and power therein and producing an electropherogram, integrating the current or the power to determine the cumulative current or power as a function of the separation time, transforming the electropherogram to a second electropherogram representing the signal as a function of the cumulative current or power, and identifying in the second electropherogram peaks that are correlated with the analytes in the sample. The invention also provides systems for performing the analysis and identification methods, as well as computer-readable products for performing the steps associated with the above methods.

US Patent:

56664354, Sep 9, 1997

Filed:

Dec 9, 1994

Appl. No.:

8/354412

Inventors:

Dean S. Burgi - Menlo Park CA
Daniel M. Bartell - Foster City CA
Donald D. Sleeter - San Leandro CA
Lucina P. Mastro - San Francisco CA

Assignee:

Genomyx Corporation - Foster City CA

International Classification:

G06K 900

US Classification:

382129

Abstract:

A system for identification of DNA bases in a nucleotide sequence includes a microprocessor and a set of executable instructions and is configured to accept a set of input data corresponding to a DNA sequence image from an autoradiograph. In part by interpolating a distribution of density peaks derived from the set of input data, the system is configured to generate a set of output data substantially identifying or presenting the bases in the DNA sequence in a linear fashion, corresponding to the linear order of nucleotides comprising the DNA sequence. The system thereby enables identification of substantially more bases than could be read by the human eye. Enhanced electrophoresis equipment and consumables may also be applied to generate a more predictable and linear set of input data and, in turn, to generate a set of output data identifying an even greater number of DNA bases in the sequence under analysis.

US Patent:

50890999, Feb 18, 1992

Filed:

Nov 30, 1990

Appl. No.:

7/621169

Inventors:

Dean S. Burgi - Palo Alto CA

Assignee:

Varian Associates, Inc. - Palo Alto CA

International Classification:

B01D 5702

US Classification:

2041801

Abstract:

An improved electroinjection method of introducing a sample having positive and negative ions into a capillary column for CE or CZE process by introducing a short plug of a low concentration buffer before introducing the sample into the column which results in increasing amount of charged ions of the sample introduced in the capillary column due to increasing the electric field at the injection point. A method for efficiently introducing both positive and negative ions is also shown.

US Patent:

51164710, May 26, 1992

Filed:

Oct 4, 1991

Appl. No.:

7/771575

Inventors:

Dean S. Burgi - Palo Alto CA

Assignee:

Varian Associates, Inc. - Palo Alto CA

International Classification:

G01N 2726
B01D 5702

US Classification:

2041801

Abstract:

The method for improving sample concentration in capillary electrophoresis by extracting the sample buffer from the capillary column using the electro-osmotic flow while the sample components are stacked into the support buffer utilizes a system for this technique. The system has a separation column 11, sample introduction means 16 for injecting the sample solution, an injection detector means 20 for detecting the volume of injected sample solution, a separation detector means 21 for detecting the sample components, power supply means 17 for applying a high voltage along the separation column 11 for reversing the direction of the electro-osmotic flow thereby causing extraction of the sample buffer from the separation column and afterward providing the separation sample into its components.

US Patent:

55601251, Oct 1, 1996

Filed:

Dec 9, 1994

Appl. No.:

8/353119

Inventors:

Dean S. Burgi - Menlo Park CA

Assignee:

Genomyx Corporation - Foster City CA

International Classification:

F26B 300

US Classification:

34508

Abstract:

A method for drying electrophoresis gel comprising, in combination, using a gaseous moisture removing medium, a gaseous moisture removing medium driving means, and an impingement means, whereby the gaseous moisture removing medium is driven by the gaseous moisture removing medium driving means across the impingement means to provide a flow of the gaseous moisture removing medium on the surface of the gel plates, whereby the flow induced by passage of the gaseous moisture removing medium through the impingement means thereby minimizes temperature gradients within the gel by forced convection and reduces drying time of the gel.

US Patent:

57360251, Apr 7, 1998

Filed:

Nov 7, 1996

Appl. No.:

8/744689

Inventors:

Douglas H. Smith - Los Altos CA
Brian J. Mifsud - San Francisco CA
Dean S. Burgi - Menlo Park CA
Thomas E. Davis - San Francisco CA
Steven M. VanHuystee - San Mateo CA

Assignee:

Genomyx Inc. - Foster City CA

International Classification:

C25B 900

US Classification:

204621

Abstract:

A gel electrophoresis separation apparatus comprising, in combination, a gaseous heat-exchange medium, a gaseous heat-exchange medium driving means, and an impingement means, whereby the gaseous heat-exchange medium is driven by the gaseous heat-exchange medium driving means across the impingement means to provide a flow of the gaseous heat-exchange medium on the surface of the gel plates, whereby the flow induced by passage of the gaseous heat-exchange medium through the impingement means thereby minimizes temperature gradients within the gel by forced convection.