Deborah Kay Hanson

US Patent:

8455231, Jun 4, 2013

Filed:

Feb 8, 2010

Appl. No.:

12/702167

Inventors:

Philip D. Laible - Villa Park IL, US
Deborah K. Hanson - Villa Park IL, US

Assignee:

Uchicago Argonne, LLC - Chicago IL

International Classification:

C12N 9/10

US Classification:

435193, 4351737, 4353071

Abstract:

The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.

US Patent:

20020102655, Aug 1, 2002

Filed:

Feb 1, 2001

Appl. No.:

09/775345

Inventors:

Philip Laible - Villa Park IL, US
Deborah Hanson - Downers Grove IL, US

International Classification:

C12P021/04
C07H021/04

US Classification:

435/069700, 435/320100, 536/023500

Abstract:

A method for expressing and purifying foreign proteins in photosynthetic organisms comprising the simultaneous expression of both the heterologous protein and a means for compartmentalizing or sequestering of the protein.

US Patent:

20070092943, Apr 26, 2007

Filed:

Sep 28, 2006

Appl. No.:

11/536331

Inventors:

Philip Laible - Villa Park IL, US
Deborah Hanson - Villa Park IL, US

Assignee:

THE UNIVERSITY OF CHICAGO - Chicago IL

International Classification:

C12P 21/06
C07H 21/04
C12N 15/74
C12N 1/21
C07K 14/195

US Classification:

435069100, 435252300, 435471000, 530350000, 536023700

Abstract:

Methods for expressing and purifying foreign (heterologous) proteins in photosynthetic organisms employ expression of both heterologous membrane proteins and a means for compartmentalizing or sequestering of the protein.

US Patent:

20140080176, Mar 20, 2014

Filed:

Sep 14, 2012

Appl. No.:

13/619406

Inventors:

Philip D. Laible - Villa Park IL, US
Deborah K. Hanson - Villa Park IL, US

Assignee:

UCHICAGO ARGONNE, LLC - Chicago IL

International Classification:

C12N 1/21
C12P 21/00
C12P 1/04
C12N 15/74
C12M 1/42

US Classification:

435 691, 4352523, 435471, 4352852, 435170

Abstract:

The invention provides an organism for expressing foreign DNA, the organism engineered to accept standard DNA carriers. The genome of the organism codes for intracytoplasmic membranes and features an interruption in at least one of the genes coding for restriction enzymes. Further provided is a system for producing biological materials comprising: selecting a vehicle to carry DNA which codes for the biological materials; determining sites on the vehicle's DNA sequence susceptible to restriction enzyme cleavage; choosing an organism to accept the vehicle based on that organism not acting upon at least one of said vehicle's sites; engineering said vehicle to contain said DNA; thereby creating a synthetic vector; and causing the synthetic vector to enter the organism so as cause expression of said DNA.