Deborah Fry Wilson

US Patent:

7445930, Nov 4, 2008

Filed:

Jul 30, 2004

Appl. No.:

10/909066

Inventors:

Shuyuan Zhang - Sugarland TX, US
Capucine Thwin - Seattle WA, US
Zheng Wu - Sugarland TX, US
Toohyon Cho - Philadelphia PA, US
Deborah Wilson - Houston TX, US
Lucetta Caston - Limerick PA, US

Assignee:

Introgen Therapeutics Inc. - Austin TX

International Classification:

C12N 15/00
A61K 39/00
A61K 39/23
A61K 39/095

US Classification:

4353201, 4241841, 4242331

Abstract:

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

US Patent:

7510875, Mar 31, 2009

Filed:

Dec 27, 2001

Appl. No.:

10/033571

Inventors:

Shuyuan Zhang - Sugarland TX, US
Capucine Thwin - Seattle WA, US
Zheng Wu - Sugarland TX, US
Toohyon Cho - Philadelphia PA, US
Deborah Wilson - Houston TX, US
Lucetta Caston - Limerick PA, US

Assignee:

Introgen Therapuetics, Inc. - Austin TX

International Classification:

C12N 7/02
C12N 5/00
A61K 39/235
C07K 1/00
A23J 1/00

US Classification:

435329, 4242331, 435383, 530417

Abstract:

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.