Debra Allen Bryan

US Patent:

20200230598, Jul 23, 2020

Filed:

Mar 23, 2020

Appl. No.:

16/827391

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

B01L 3/00
G01N 35/10
B01F 15/02
B01F 13/00
B01F 11/00

Abstract:

An analysis system may perform operations on an analyte that may be combined with multiple regents prior to being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel. For this, the reagents may be aspirated from a recipient into the volume or channel, ejected back into the recipient, and this process may be performed repeatedly to enhance mixing

US Patent:

20180185842, Jul 5, 2018

Filed:

Dec 13, 2017

Appl. No.:

15/841102

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

B01L 3/00

Abstract:

An analysis system may perform operations on an analyte that may be combined with multiple regents prior to being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel. For this, the reagents may be aspirated from a recipient into the volume or channel, ejected back into the recipient, and this process may be performed repeatedly to enhance mixing

US Patent:

20180188141, Jul 5, 2018

Filed:

Dec 13, 2017

Appl. No.:

15/841098

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
James Michael Osmus - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

G01N 1/38
G01N 1/14
B01L 3/00
G01N 35/10
C12Q 1/6874
B01F 13/10
B01F 15/02
B01F 5/00
B01F 5/06
B01F 3/08

Abstract:

An analysis instrument may perform analytical operations on an analyte that is combined with multiple reagents prior to being introduced into a flow cell. The instrument may include a nozzle sipper that aspirates reagents from a recipient, along with an analyte. The reagents may be directed to a volume and may be repeatedly moved into and out of the volume by cycling of a pump. The reagents may be ejected into a destination recipient with the nozzle sipper promoting vorticity in the recipient to enhance mixing. The repeated aspiration and ejection through the nozzle sipper effectively mixes the reagents and the template in an automated or semi-automated fashion.