Donald W. Mercer - Pittsburgh PA Edward C. Horey - Pittsburgh PA
Assignee:
Donbee Corporation - Pittsburgh PA
International Classification:
A63F 504
US Classification:
273141R
Abstract:
A board game simulating an athletic contest utilizes a plurality of spinner cards to provide the players with options. The spinner cards carry indicia associating them with positions on the game board corresponding to positions of the contestants on the playing field. A spinner assembly having a spinner disc, a spinner shaft and a pivotally mounted pointer arm is adapted for demountable securement to a number of spinner cards.
Assay Of Isoenzymes By Ion Exchange Chromatography
A simple, rapid anion-exchange column chromatographic technique is used to determine the presence of fractions of proteins and enzymes in human serum by separating the fractions on the basis of their net electrical charges. Samples of serum of unaltered ionic strength, layered on mini columns of an ion exchange agent previously equilibrated to a low ionic strength similar to blood serum with a buffer solution, are eluted stepwise with an appropriate buffer solution having different ionic strengths at each step. Elution of the samples is without previous dialysis or other ionization treatment. Column effluents are assayed for protein content or enzyme activity by conventional means, e. g. , spectrophotometric analysis. Evaluation of sera from patients utilizing these methods reveals protein fractions or isoenzyme patterns which identify specific diseases associated with the protein or isoenzyme pattern.