Dong Cai

US Patent:

20070231908, Oct 4, 2007

Filed:

Sep 22, 2005

Appl. No.:

11/233481

Inventors:

Dong Cai - Cambridge MA, US
David Carnahan - Needham MA, US

International Classification:

C12N 15/87

US Classification:

435459000, 977906000

Abstract:

A nanostructured molecular delivery vehicle comprising magnetic materials and configured to receive passenger biomolecules. The application of a an appropriate magnetic field having a gradient orients and drives the vehicle into a biological target, which may comprise cells, cell masses, tissue slices, tissues, etc. Under the control of the magnetic field, these vehicles can penetrate cell membranes. Then, the biomolecules carried by the vehicle can be released into the cells to perform their functions. Using this “nanospearing” technique, unprecendented high transfection efficiency has been achieved in several difficult-to-transfect cells. These include, but are not limited to, Bal 17 cells, ex vivo B cells, primary cultured cortical neurons, etc. This method advances the state of the art, providing an improved technique for the introduction of exogenous molecules to cells, with the clinical applications including, but not being limited to, drug delivery, gene therapy, vaccination, etc.

US Patent:

20110287977, Nov 24, 2011

Filed:

Nov 16, 2007

Appl. No.:

12/514689

Inventors:

Dong Cai - Cambridge MA, US
Thomas Chiles - Norfolk MA, US
Krzysztof Kempa - Chestnut Hill MA, US
Michael Naughton - Norwood MA, US
Zhifeng Ren - Newton MA, US
Paudel Trilochan - Allston MA, US

International Classification:

C40B 40/00
G01N 30/96
G01N 27/00
B82Y 15/00

US Classification:

506 13, 422 8201, 422 69, 977742, 977957, 977752

Abstract:

A nanocoaxial sensor includes an outer conductor, an inner conductor, a dielectric material disposed between the outer and inner conductors, a nanocavity sized to allow target species to enter the nanocavity between the outer and inner conductors, and an active sensing element immobilized within the nanocavity on at least one of the inner or outer conductors. The active sensing element is adapted to selectively capture the at least one of the target species.

US Patent:

20120118751, May 17, 2012

Filed:

Feb 3, 2010

Appl. No.:

13/147526

Inventors:

Dong Cai - West Newton MA, US
Thomas C. Chiles - Norfolk MA, US

International Classification:

G01N 27/26
C25D 5/02
B82Y 15/00
B82Y 99/00
B82Y 40/00
B82Y 30/00

US Classification:

205122, 204400, 20440301, 2057805, 977832, 977742, 977789, 977773, 977762, 977897, 977899, 977902

Abstract:

An apparatus for detecting the presence of a target molecule is disclosed which includes a conductive nanostructure, a non-conductive polymer coating on at least a portion of the nanostructure, and a cavity formed in the polymer coating having a shape corresponding to the shape of the target molecule. A property of the nanostructure depends on the presence of the target molecule at the cavity.

US Patent:

20140015548, Jan 16, 2014

Filed:

Mar 21, 2012

Appl. No.:

14/005205

Inventors:

Michael J. Naughton - Chestnut Hill MA, US
Dong Cai - West Newton MA, US
Binod Rizal - Brighton MA, US
Thomas Chiles - Norfolk MA, US
Huaizhou Zhao - Brighton MA, US

International Classification:

G01R 27/26

US Classification:

324658, 29829

Abstract:

A nanocoaxial sensor includes an outer conductor, an inner conductor, a nanoporous dielectric material disposed between the outer and inner conductors, a nanocavity sized to allow target species to enter the nanocavity between the outer and inner conductors.

US Patent:

20180369140, Dec 27, 2018

Filed:

Jul 9, 2018

Appl. No.:

16/029809

Inventors:

- Houston TX, US
Dong CAI - Houston TX, US

International Classification:

A61K 9/00
A61K 47/69
A61K 48/00
A61K 33/24
A61K 31/713
A61K 31/555
A61K 31/519
A61K 31/495
A61K 31/4745
A61K 31/175
A61K 41/00
A61K 31/337
A61K 9/50

Abstract:

A method of selectively targeting a cell with a therapeutic agent, the method comprising: targeting a cell with a nanospear, puncturing the cell with said nanospear; releasing a therapeutic agent from said nanospear, wherein said therapeutic agent enters said cell, thereby effecting the efficacy of said cell.

US Patent:

20170258714, Sep 14, 2017

Filed:

May 1, 2017

Appl. No.:

15/583274

Inventors:

- Houston TX, US
Dong CAI - Houston TX, US

Assignee:

University of Houston System - Houston TX

International Classification:

A61K 9/00
A61K 48/00
A61K 41/00

Abstract:

A method of selectively targeting a cell with a therapeutic agent, the method comprising: targeting a cell with a nanospear, puncturing the cell with said nanospear; releasing a therapeutic agent from said nanospear, wherein said therapeutic agent enters said cell, thereby effecting the efficacy of said cell.

US Patent:

20170233435, Aug 17, 2017

Filed:

Aug 4, 2015

Appl. No.:

15/501796

Inventors:

Zhifeng REN - Houston TX, US
Dong CAI - Houston TX, US
Zhen YANG - Houston TX, US
- Houston TX, US

International Classification:

C07K 1/14

Abstract:

A method of extracting biomolecules from live cells comprising: introducing a plurality of magnetized carbon nanotubes (MCNTs) into a live cell, wherein the MCNTs penetrate the cell membrane under a magnetic force; spearing the MCNTs through the cell under the magnetic force, wherein a biomolecule attaches to at least a portion of the MCNTs to form a biomolecule loaded MCNT; removing at least a portion of the biomolecule loaded MCNTs from the cell under the magnetic force; and collecting at least a portion of the biomolecule loaded MCNTs.