Donna M Leippe

US Patent:

6335162, Jan 1, 2002

Filed:

Mar 13, 1998

Appl. No.:

09/042287

Inventors:

John W. Shultz - Verona WI
Michelle A. Nelson - Fitchburg WI
Donna M. Leippe - Madison WI
Martin K. Lewis - Madison WI
Lisa S. Nelson - DeForest WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168

US Classification:

435 6, 435 911

Abstract:

This invention discloses methods, compositions and kits for the detection of extremely low levels of nucleic acid, cells and cellular material in biological samples. The nucleic acid detection systems utilize either the pyrophosphorolysis reaction catalyzed by various polymerases or nuclease digestion coupled with pyrophosphorylation catalyzed by phosphoribosylpyrophosphate synthetase to produce either deoxyribonucleoside triphosphates or ribonucleoside triphosphates. dNTPs are transformed to ATP by the action of nucleoside diphosphate kinase. The ATP produced by these reactions may be detected by luciferase or NADH based detection systems. If more sensitive detection is required, schemes for the amplification of NTPs and dNTPS are provided. A detection system for cells or cellular material in a sample is provided wherein AMP and a high energy phosphate donor added to a sample are converted to ATP by the action of endogenous enzymes, followed by detection of the ATP.

US Patent:

6379898, Apr 30, 2002

Filed:

Jan 9, 2001

Appl. No.:

09/757132

Inventors:

John W. Shultz - Verona WI 53593
Michelle A. Nelson - Fitchburg WI 53713
Donna M. Leippe - Madison WI 53705
Martin K. Lewis - Madison WI 53716
Lisa S. Nelson - DeForest WI 53532

International Classification:

C12Q 168

US Classification:

435 6, 435 71

Abstract:

This invention discloses methods, compositions and kits for the detection of extremely low levels of nucleic acid, cells and cellular material in biological samples. The nucleic acid detection systems utilize either the pyrophosphorolysis reaction catalyzed by various polymerases or nuclease digestion coupled with pyrophosphorylation catalyzed by phosphoribosylpyrophosphate synthetase to produce either deoxyribonucleoside triphosphates or ribonucleoside triphosphates. dNTPs are transformed to ATP by the action of nucleoside diphosphate kinase. The ATP produced by these reactions may be detected by luciferase or NADH based detection systems. If more sensitive detection is required, schemes for the amplification of NTPs and dNTPS are provided. A detection system for cells or cellular material in a sample is provided wherein AMP and a high energy phosphate donor added to a sample are converted to ATP by the action of endogenous enzymes, followed by detection of the ATP.

US Patent:

6391551, May 21, 2002

Filed:

Aug 25, 1999

Appl. No.:

09/383316

Inventors:

John William Shultz - Verona WI
Martin K. Lewis - Madison WI
Donna Leippe - Madison WI
Michelle Mandrekar - Oregon WI
Daniel Kephart - Cottage Grove WI
Richard Byron Rhodes - Madison WI
Christine Ann Andrews - Cottage Grove WI
James Robert Hartnett - Madison WI
Trent Gu - Madison WI
Ryan J. Olson - Madison WI
Keith V. Wood - Madison WI
Roy Welch - Palo Alto CA

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168

US Classification:

435 6, 536 2431, 536 2432

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

US Patent:

6653078, Nov 25, 2003

Filed:

Feb 20, 2001

Appl. No.:

09/788847

Inventors:

Martin K. Lewis - Madison WI
Daniel Kephart - Cottage Grove WI
Richard Byron Rhodes - Madison WI
John William Shultz - Verona WI
Donna Leippe - Madison WI
Michelle Mandrekar - Oregon WI
Christine Ann Andrews - Cottage Grove WI
James Robert Hartnett - Madison WI
Trent Gu - Madison WI
Keith V. Wood - Madison WI
Roy Welch - Palo Alto CA

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168

US Classification:

435 6, 435 691, 435214, 536 221, 536 231, 536 235, 536 29, 536 24, 536 26

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of predetermined nucleic acid target sequences using a multiplex assay format. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, genotyping, medical marker diagnostics, and the like.

US Patent:

6730479, May 4, 2004

Filed:

Feb 22, 2001

Appl. No.:

09/790417

Inventors:

John William Shultz - Verona WI
Martin K. Lewis - Madison WI
Donna Leippe - Madison WI
Michelle Mandrekar - Oregon WI
Daniel Kephart - Cottage Grove WI
Richard Byron Rhodes - Madison WI
Christine Ann Andrews - Cottage Grove WI
James Robert Hartnett - Madison WI
Trent Gu - Madison WI
Ryan J. Olson - Madison WI
Keith V. Wood - Madison WI
Roy Welch - Palo Alto CA

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168

US Classification:

435 6, 4353201, 4352528, 435174, 435183, 536 237, 530350, 935 10, 935 24, 935 72

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

US Patent:

20030077621, Apr 24, 2003

Filed:

May 20, 2002

Appl. No.:

10/152297

Inventors:

John Shultz - Verona WI, US
Martin Lewis - Madison WI, US
Donna Leippe - Middleton WI, US
Michelle Mandrekar - Oregon WI, US
Daniel Kephart - Cottage Grove WI, US
Richard Rhodes - Madison WI, US
Christine Andrews - Cottage Grove WI, US
James Hartnett - Madison WI, US
Trent Gu - Madison WI, US
Ryan Olson - Middleton WI, US
Keith Wood - Madison WI, US
Roy Welch - Palo Alto CA, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, spieciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

US Patent:

20030203358, Oct 30, 2003

Filed:

Feb 9, 2001

Appl. No.:

09/780863

Inventors:

John Shultz - Verona WI, US
Martin Lewis - Madison WI, US
Donna Leippe - Madison WI, US
Michelle Mandrekar - Oregon WI, US
Daniel Kephart - Cottage Grove WI, US
Richard Rhodes - Madison WI, US
Christine Andrews - Cottage Grove WI, US
James Hartnett - Madison WI, US
Trent Gu - Madison WI, US
Ryan Olson - Madison WI, US
Keith Wood - Madison WI, US
Roy Welch - Palo Alto CA, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.

US Patent:

20050214753, Sep 29, 2005

Filed:

Feb 22, 2001

Appl. No.:

09/790457

Inventors:

John Shultz - Verona WI, US
Martin Lewis - Madison WI, US
Donna Leippe - Madison WI, US
Michelle Mandrekar - Oregon WI, US
Daniel Kephart - Cottage Grove WI, US
Richard Rhodes - Madison WI, US
Christine Andrews - Cottage Grove WI, US
James Hartnett - Madison WI, US
Trent Gu - Madison WI, US
Ryan Olson - Madison WI, US
Keith Wood - Madison WI, US
Roy Welch - Palo Alto CA, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.