Douglas J South

US Patent:

20130217026, Aug 22, 2013

Filed:

Feb 21, 2013

Appl. No.:

13/773350

Inventors:

LOCKHEED MARTIN CORPORATION - , US
Orion N. Scott - Charlottesville VA, US
Douglas J. South - Rockville MD, US
Joan Bienvenue - Fredericksburg VA, US
Paul Kinnon - Solana Beach CA, US
James Landers - Charlottesville VA, US
David Saul - Auckland, NZ
An-Chi Tsuei - Charlottesville VA, US
Jason Hayes - Heathmont, AU
Matthew Springer - West Melbourne, AU
Matthew Solomon - Hughesdale, AU
Peter van Ruijven - Glen Waverley, AU

Assignee:

MICROLAB DIAGNOSTICS, INC. - Charlottesville VA
LOCKHEED MARTIN CORPORATION - BETHESDA MD

International Classification:

C12Q 1/68

US Classification:

435 612, 4352872

Abstract:

A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.

US Patent:

20200002745, Jan 2, 2020

Filed:

Feb 7, 2019

Appl. No.:

16/270218

Inventors:

- Bethesda MD, US
- Hamilton, NZ
James LANDERS - Charlottesville VA, US
Douglas J. SOUTH - Rockville MD, US
Brian E. ROOT - Charlottesville VA, US

Assignee:

LOCKHEED MARTIN CORPORATION - Bethesda MD
ZYGEM CORPORATION LTD. - Hamilton

International Classification:

C12Q 1/686

Abstract:

A cartridge interface module (CIM), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The CIM also includes a polymerase chain reaction (PCR) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids. The CIM may also include a high voltage electrodes component that is configured to initiate and support separation of the amplified nucleic acids into nucleic acid fragments in a separation channel of the removable microfluidic cartridge. The CIM also includes a detection optics component that can be configured to collect, detect, and direct label nucleic acid fragments. The CIM is configured to integrate with a microfluidic chip architecture of an inserted removable microfluidic cartridge.

US Patent:

20150284775, Oct 8, 2015

Filed:

Jun 19, 2015

Appl. No.:

14/744889

Inventors:

- Bethesda MD, US
- Charlottesville VA, US
Orion N. Scott - Charlottesville VA, US
Douglas J. South - Rockville MD, US
Joan M. Bienvenue - Fredericksburg VA, US
Paul Kinnon - Solana Beach CA, US
James Landers - Charlottesville VA, US
David Saul - Auckland, NZ
An-Chi Tsuei - Charlottesville VA, US
Jason Hayes - Heathmont, AU
Matthew Springer - West Melbourne, AU
Matthew Solomon - Hughesdale, AU
Peter Van Ruijven - Glen Waverley, AU

Assignee:

LOCKHEED MARTIN CORPORATION - Bethesda MD
MICROLAB DIAGNOSTICS, INC. - Charlottesville VA

International Classification:

C12Q 1/68
B01L 7/00
B01L 3/00

Abstract:

A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.

US Patent:

20140295441, Oct 2, 2014

Filed:

Mar 27, 2014

Appl. No.:

14/227645

Inventors:

- Hamilton, NZ
- Bethesda MD, US
James Landers - Charlottesville VA, US
Douglas J. South - Rockville MD, US
Brian E. Root - Charlottesville VA, US

Assignee:

ZYGEM CORPORATION LTD. - Hamilton
LOCKHEED MARTIN CORPORATION - Bethesda MD

International Classification:

C12Q 1/68

US Classification:

435 612, 4352872

Abstract:

A cartridge interface module (CIM), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The CIM also includes a polymerase chain reaction (PCR) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids. The CIM may also include a high voltage electrodes component that is configured to initiate and support separation of the amplified nucleic acids into nucleic acid fragments in a separation channel of the removable microfluidic cartridge. The CIM also includes a detection optics component that can be configured to collect, detect, and direct label nucleic acid fragments. The CIM is configured to integrate with a microfluidic chip architecture of an inserted removable microfluidic cartridge.