Edward J Dougherty

US Patent:

7247426, Jul 24, 2007

Filed:

Aug 2, 2001

Appl. No.:

09/921406

Inventors:

Zohar Yakhini - Yaacov, IL
Michael Bittner - Rockville MD, US
Jeff Trent - Rockville MD, US
Amir Ben-Dor - Bellevue WA, US
Paul Meltzer - Rockville MD, US
Yidong Chen - Rockville MD, US
Ashani Weeraratna - Owings Mil MD, US
Yuan Jiang - Gaithersburg MD, US
Nick Sampas - San Jose CA, US
Edward Dougherty - College Station TX, US

Assignee:

Agilent Technologies, Inc. - Santa Clara CA

International Classification:

C12Q 1/68

US Classification:

435 6, 436 64, 436501, 436518

Abstract:

The overexpression of certain marker genes including Wnt5a has been found useful in the identification of more aggressive forms of malignant melanoma. Therefore, the overexpression of these genes in tumor samples of malignant melanoma may be useful in the diagnosis, profiling, and treatment of patients suffering from this disease. Inhibitors of Wnt5a activity may be useful in the treatment of aggressive forms of malignant melanoma. Inhibition of Wnt5a activity may be effected by any method including anti-sense therapy, gene therapy, and pharmaceutical intervention.

US Patent:

51322200, Jul 21, 1992

Filed:

Jun 30, 1989

Appl. No.:

7/373977

Inventors:

Martin Shapiro - Columbia MD
Dwight E. Lynn - Severn MD
Edward M. Dougherty - Fulton MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 700

US Classification:

4352351

Abstract:

A method has been found to select the most virulent biotypes from a wild-type entomopathogenic virus by selection of desirable characteristics after serial passages through in vivo and in vitro systems. Using this method, a strain of Lymantria dispar NPV virus was isolated which had increased biological activity and increased speed of kill.

US Patent:

61435651, Nov 7, 2000

Filed:

Aug 22, 1997

Appl. No.:

8/916265

Inventors:

Edward M. Dougherty - Alexandria VA
Terry A. McKelvey - Gaithersburg MD
Donald A. Stoltz - Halifax, CA
Dwight E. Lynn - Severn MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 1587
C12N 510

US Classification:

435456

Abstract:

A stable transformed insect cell line has been achieved by infecting an insect cell culture with fluid obtained from a female parasitoid wasp, such as Glyptapanteles indiensis. The fluid contains polydnavirus DNA capable of integrating into the host cell genome. Susceptible insect cells include both Lepidopteran and Coleopteran cells.

US Patent:

51241498, Jun 23, 1992

Filed:

Nov 7, 1990

Appl. No.:

7/609848

Inventors:

Martin Shapiro - Columbia MD
Edward Dougherty - Alexandria VA
John J. Hamm - Tifton GA

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C09B 2600
A01N 2500
A01N 6300
A61K 3748

US Classification:

424 93T

Abstract:

The present invention relates to compositions and methods of their use for the management or biocontrol on insect pests. The compositions comprise an entomopathogen and a chitin synthetase inhibitor alone or in combination with a UV protectant. Alternatively, compositions are disclosed comprising an entomopathogen and a fluorescent brightener of the stilbene type which may have chitin synthetase inhibitory properties. Specifically, entomopathogens such as Nuclear polyhedrosis viruses (NPV), Cytoplasmic polyhedrosis viruses (CPV), Entomopox viruses (EPV) and Granulosis viruses (GV) and iridescent virus (IV) are protected from UV radiation and their biological activity enhanced using fluorescent brighteners analogs, particularly, 4, 4'-diamino-2, 2'-stilbene disulfonic acid.

US Patent:

62455176, Jun 12, 2001

Filed:

Sep 28, 1999

Appl. No.:

9/407021

Inventors:

Yidong Chen - Rockville MD
Edward R. Dougherty - College Station TX
Michael L. Bittner - Rockville MD

Assignee:

The United States of America as represented by the Department of Health and
Human Services - Washington DC

International Classification:

C12Q 168
G01N 3348
G01N 3100
G06F 1500

US Classification:

435 6

Abstract:

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA micro-array. Comparison of gene expression levels arising from co-hybridized samples is achieved by taking ratios of average expression levels for individual genes. In an image-processing phase, a method of image segmentation identifies cDNA target sites in a cDNA micro-array image. The resulting cDNA target sites are analyzed based on a hypothesis test and confidence interval to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.