Edward M Nolan

US Patent:

6654636, Nov 25, 2003

Filed:

Jul 26, 2000

Appl. No.:

09/625825

Inventors:

Nagendu B. Dev - San Diego CA
Gunter A. Hofmann - San Diego CA
Edward Nolan - San Diego CA
Dietmar P. Rabussay - San Diego CA
Arnt Tonnessen - El Cajon CA
Georg Widera - Del Mar CA
Lei Zhang - San Diego CA

Assignee:

Genetronics, Inc. - San Diego CA

International Classification:

A61N 130

US Classification:

604 21, 604501

Abstract:

The present invention describes an in vivo method, using pulsed electric field to deliver therapeutic agents into cells of the skin and muscle for local and systemic treatments. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.

US Patent:

6678556, Jan 13, 2004

Filed:

Jun 7, 2002

Appl. No.:

10/165657

Inventors:

Edward Nolan - San Diego CA
Dietmar P. Rabussay - Solana Beach CA
Georg Widera - Del Mar CA
Lei Zhang - San Diego CA

Assignee:

Genetronics, Inc. - San Diego CA

International Classification:

A61N 130

US Classification:

604 21, 604501

Abstract:

In vivo methods are provided for using an electric field to delivery therapeutic treatment to a subject while reducing inducement of histopathological change in the target muscle tissue, such as is associated with induction or amplification of an immune response caused by the pulsed electric field. Therapeutic agents can be delivered nto cells of muscle for local and systemic treatments with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.

US Patent:

6678558, Jan 13, 2004

Filed:

Mar 23, 2000

Appl. No.:

09/535683

Inventors:

Steve Dimmer - Valley Center CA
Gunter Hofmann - San Diego CA
Daniel Holt - Mira Mesa CA
Gurvinder Nanda - San Diego CA
Edward M. Nolan - San Diego CA

Assignee:

Genetronics, Inc. - San Diego CA

International Classification:

A61N 118

US Classification:

607 3, 607 63, 604 20

Abstract:

A method for delivery of an agent to a cell using electroporation is disclosed. The method includes positioning a first electrode and a second electrode such that an electrical signal passed between the first electrode and the second electrode passes through the cell. The method also includes passing an electrical signal between the first electrode and the second electrode, the electrical signal having a frequency greater than about 10 kHz. In one embodiment of the method, the electrical signal has a bipolar waveform. In another embodiment of the method, the electrodes are positioned at a treatment site, e. g. , a tumor, for in vivo delivery of an agent.

US Patent:

6697669, Feb 24, 2004

Filed:

Jul 13, 1999

Appl. No.:

09/352809

Inventors:

Nagendu B. Dev - San Diego CA
Gunter A. Hofmann - San Diego CA
Edward Nolan - San Diego CA
Dietmar P. Rabussay - Solana Beach CA
Arnt Tonnessen - El Cajon CA
Georg Widera - Del Mar CA
Lei Zhang - San Diego CA

Assignee:

Genetronics, Inc.

International Classification:

A61N 130

US Classification:

604 21

Abstract:

The present invention describes an in vivo method, using pulsed electric field to deliver therapeutic agents into cells of the skin and muscle for local and systemic treatments. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.

US Patent:

7054685, May 30, 2006

Filed:

Aug 8, 2002

Appl. No.:

10/215963

Inventors:

Steve Dimmer - Valley Center CA, US
Gunter Hofmann - San Diego CA, US
Daniel Holt - Mira Mesa CA, US
Gurvinder Nanda - San Diego CA, US
Edward M. Nolan - San Diego CA, US

Assignee:

Genetronics, Inc. - San Diego CA

International Classification:

A61N 1/362

US Classification:

607 3

Abstract:

A method for delivery of an agent to a cell using electroporation is disclosed. The method includes positioning a first electrode and a second electrode such that an electrical signal passed between the first electrode and the second electrode passes through the cell. The method also includes passing an electrical signal between the first electrode and the second electrode, the electrical signal having a frequency greater than about 10 kHz. In one embodiment of the method, the electrical signal has a bipolar waveform. In another embodiment of the method, the electrodes are positioned at a treatment site, e. g. , a tumor, for in vivo delivery of an agent.

US Patent:

7570992, Aug 4, 2009

Filed:

Jan 13, 2004

Appl. No.:

10/756946

Inventors:

Edward Nolan - San Diego CA, US
Dietmar P. Rabussay - Solana Beach CA, US
Georg Widera - Del Mar CA, US
Lei Zhang - San Diego CA, US

Assignee:

Genetronics, Inc. - San Diego CA

International Classification:

A61N 1/30
A61M 31/00

US Classification:

604 21, 604501

Abstract:

In vivo methods are provided for using an electric field to delivery therapeutic treatment to a subject while reducing inducement of histopathological change in the target muscle tissue, such as is associated with induction or amplification of an immune response caused by the pulsed electric field. Therapeutic agents can be delivered nto cells of muscle for local and systemic treatments with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.

US Patent:

20020019052, Feb 14, 2002

Filed:

Oct 10, 2001

Appl. No.:

09/974882

Inventors:

Edward Nolan - San Diego CA, US
Dietmar Rabussay - Solana Beach CA, US
Gunter Hofmann - San Diego CA, US

Assignee:

GENETRONICS, INC.

International Classification:

C12N015/87
A61K048/00

US Classification:

435/461000, 514/044000

Abstract:

The present invention provides methods and apparatus for the delivery of at least one chromosome into a cell. Invention methods and apparatus employ FACS or MACS technology for rapidly processing cells and for confirming the introduction of chromosome(s) into a cell. The introduction of the chromosome(s) into the cell is mediated by one or more of a laser, a linear accelerator or electrically induced fusion of a cell and encapsulated chromosome(s). Invention methods provide for the rapid and reliable processing associated with FACS and MACS technology to process thousands of cells a minute, thereby enabling large scale gene transfer.

US Patent:

20040092860, May 13, 2004

Filed:

Jun 9, 2003

Appl. No.:

10/457894

Inventors:

Nagendu Dev - San Diego CA, US
Gunter Hofmann - San Diego CA, US
Edward Nolan - San Diego CA, US
Dietmar Rabussay - San Diego CA, US
Arnt Tonnessen - El Cajon CA, US
Georg Widera - Del Mar CA, US
Lei Zhang - San Diego CA, US

International Classification:

A61N001/30

US Classification:

604/021000

Abstract:

The present invention describes an in vivo method, using pulsed electric field to deliver therapeutic agents into cells of the skin and muscle for local and systemic treatments. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.