Edward W Rossi

US Patent:

20090111143, Apr 30, 2009

Filed:

Oct 24, 2007

Appl. No.:

11/877728

Inventors:

David M. Goldenberg - Mendham NJ, US
Chien-Hsing Chang - Downingtown PA, US
Edward A. Rossi - Nutley NJ, US
Diane Nordstrom - Rockaway NJ, US

Assignee:

Immunomedics, Inc. - Morris Plains NJ

International Classification:

C12P 21/00
C12N 5/00
C07K 14/00
C07K 16/00
C07K 14/54
C07K 14/555
C07K 14/475
C07K 14/575
C07K 14/52
C12N 9/00
C07K 14/505

US Classification:

435 694, 435325, 530350, 5303871, 5303873, 530399, 530351, 435183, 530397, 435 691, 435 696, 435 695, 435 6951, 435 6952

Abstract:

Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transfection under serum-free conditions. In preferred embodiments, the cells of use are SpESF or SpESF-X cells.

US Patent:

20130273804, Oct 17, 2013

Filed:

Apr 16, 2012

Appl. No.:

13/447944

Inventors:

Edward S. Rossi - Montvale NJ, US
Aquiles Mata - Austin TX, US

Assignee:

In The Box Entertainment, LLC - Park Ridge NJ

International Classification:

A63H 13/16

US Classification:

446310

Abstract:

An entertainment device includes a box, a crank, an interchangeable toy, and an interior spring arranged so that turning the crank causes the toy to pop out of the box at a present time under the influence of the spring. A custom mount can be attached to the interchangeable toy, while being mounted on the top of the spring. The toy can be any type of toy, e.g., a doll, truck, baseball, and so forth. Unlike a conventional jack-in-the-box where a toy is permanently attached to the spring, the toy of the present invention is attached to the spring though the custom mount and thus, is readily removable for additional play value. As a result, the entertainment device can be customized to suit the preference of the user by simply exchanging the toy.