Edwin K Oto

US Patent:

58518185, Dec 22, 1998

Filed:

Mar 28, 1997

Appl. No.:

8/827236

Inventors:

Shi Kun Huang - Castro Valley CA
Edwin Kiyoshi Oto - Redwood City CA
Mohammad Hassanipour - Vallejo CA
Bei Jin - Union City CA

Assignee:

Sequus Pharmaceuticals, Inc. - Menlo Park CA

International Classification:

C12N 1564
A01N 2526
B01J 1302
C12P 2102

US Classification:

4353201

Abstract:

An improvement in a method of preparing plasmid-liposome complexes for in vivo transfection is described. The improvement includes selecting a condensing agent to condense the plasmid prior to contact with the liposomes, selecting a working medium and selecting a ratio of liposome lipid to plasmid. Also disclosed are DNA plasmid-liposome complexes formed by the method.

US Patent:

61330264, Oct 17, 2000

Filed:

Sep 11, 1998

Appl. No.:

9/151436

Inventors:

Shi Kun Huang - Castro Valley CA
Edwin Kiyoshi Oto - Redwood City CA
Mohammad Hassanipour - Vallejo CA
Bei Jin - Union City CA

Assignee:

Sequus Pharmaceuticals, Inc. - Menlo Park CA

International Classification:

C12N 1563
A01N 2526
C07H 2104
C12P 2102

US Classification:

4353201

Abstract:

A plasmid-liposome composition for transfection of a cell is described. The composition includes plasmid molecules condensed with a polycationic condensing agent and cationic liposomes. Also disclosed is a method for preparing the plasmid-liposome complexes.