Eileen Marie Wilson

US Patent:

7569391, Aug 4, 2009

Filed:

Dec 6, 2006

Appl. No.:

11/634737

Inventors:

Brian M. Beyer - Lincroft NJ, US
Bruce A. Malcolm - Paoli PA, US
Corey O. Strickland - Martinsville NJ, US
Wenyan Wang - Edison NJ, US
Eileen Wilson - Raritan NJ, US

Assignee:

Schering Corporation - Kenilworth NJ

International Classification:

G01N 31/00
G01N 33/00

US Classification:

436 4, 435183, 117927

Abstract:

The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding human BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.

US Patent:

8063185, Nov 22, 2011

Filed:

May 4, 2009

Appl. No.:

12/434897

Inventors:

Brian M. Beyer - Matawan NJ, US
Bruce A. Malcolm - Paoli PA, US
Corey O. Strickland - Martinsville NJ, US
Wenyan Wang - Edison NJ, US
Eileen Wilson - Raritan NJ, US

Assignee:

Schering Corp. - Kenilworth NJ

International Classification:

C12N 1/12

US Classification:

530380

Abstract:

The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.

US Patent:

7166454, Jan 23, 2007

Filed:

May 22, 2003

Appl. No.:

10/443949

Inventors:

Brian M. Beyer - Lincroft NJ, US
Bruce A. Malcolm - Westfield NJ, US
Corey O. Strickland - Martinsville NJ, US
Wenyan Wang - Edison NJ, US
Eileen Wilson - Raritan NJ, US

Assignee:

Schering Corporation - Kenilworth NJ

International Classification:

C12N 9/48
C12N 15/00
C12N 1/20
C12N 1/14
C12N 5/10
C12N 5/00
C07H 21/04

US Classification:

435212, 536 232, 4353201, 4352523, 43525411, 435419, 435325, 43525233

Abstract:

The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. Polypeptides used herein are derived from human BACE which is also known by the synonyms “mamapsin 2”, “human β-site APP-cleaving enzyme, and Asp2”. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.