Elaine M Rose

US Patent:

20110059455, Mar 10, 2011

Filed:

Sep 2, 2010

Appl. No.:

12/874602

Inventors:

Feng Yang - Acton MA, US
Sha-Sha Wang - Wellesley MA, US
Laurence Michael Vaughan - Cockeysville MD, US
Michael Porter - Baltimore MD, US
Elaine Rose - Raleigh NC, US

Assignee:

BECTON, DICKINSON AND COMPANY - Franklin Lakes NJ

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435 6, 435 792

Abstract:

A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

US Patent:

20220372554, Nov 24, 2022

Filed:

Aug 8, 2022

Appl. No.:

17/883403

Inventors:

- Franklin Lakes NJ, US
Sha-Sha Wang - Wellesley MA, US
Laurence Michael Vaughan - Cockeysville MD, US
Michael Porter - Baltimore MD, US
Elaine Rose - Raleigh NC, US

International Classification:

C12Q 1/6806
C12N 1/06
C12Q 1/70
G01N 33/569

Abstract:

A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

US Patent:

20190256893, Aug 22, 2019

Filed:

Apr 30, 2019

Appl. No.:

16/399305

Inventors:

- Franklin Lakes NJ, US
Sha-Sha Wang - Wellesley MA, US
Laurence Michael Vaughan - Cockeysville MD, US
Michael Porter - Baltimore MD, US
Elaine Rose - Raleigh NC, US

International Classification:

C12Q 1/6806
G01N 33/569
C12N 1/06
C12Q 1/70

Abstract:

A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

US Patent:

20160194687, Jul 7, 2016

Filed:

Mar 11, 2016

Appl. No.:

15/067428

Inventors:

- Franklin Lakes NJ, US
Sha-Sha Wang - Wellesley MA, US
Laurence Michael Vaughan - Cockeysville MD, US
Michael Porter - Baltimore MD, US
Elaine Rose - Raleigh NC, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q 1/68
G01N 33/569
C12Q 1/70
C12N 1/06

Abstract:

A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.