Elizabeth J Light

US Patent:

7087379, Aug 8, 2006

Filed:

May 22, 2001

Appl. No.:

09/863125

Inventors:

Elizabeth S. Light - Gaithersburg MD, US

Assignee:

Ventana Medical Systems, Inc. - Tuscan AZ

International Classification:

C12Q 1/68
C12M 3/00
C07H 21/04

US Classification:

435 6, 4352872, 536 231, 536 243, 436 94, 436164

Abstract:

A method for detecting single copies of a gene in-situ using brightfield microscopy is used in detection of nucleic acid sequences. Probes are directly or indirectly labeled with alkaline phosphatase with NBT/BCIP used as the chromogen.

US Patent:

7569344, Aug 4, 2009

Filed:

Aug 22, 2003

Appl. No.:

10/646633

Inventors:

Elizabeth S. Light - Gaithersburg MD, US
Gerard J. Nuovo - Westerville OH, US

Assignee:

Ventana Medical Systems, Inc. - Tuscon AZ

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 435 911, 536 231, 536 2432, 536 243

Abstract:

Methods and reagents for detecting high-risk human papilloma virus (HPV) DNA types in cells on a Pap smear that indicates the patient is at higher risk for cancer are described. The method uses full-length DNA probes to HPV types 16, 18, 31, 33, 35, and 51 in a particular proportion to hybridize to and detect the viral DNA in situ. The method differentiates high-risk from low-risk HPV DNA in cells that indicates the patient's risk for cancer. The in situ hybridization is detected by brightfield microscopy.

US Patent:

20060275784, Dec 7, 2006

Filed:

Apr 1, 2006

Appl. No.:

11/278410

Inventors:

Elizabeth Light - Gaithersburg MD, US
Gerard Nuovo - Lewis Center OH, US
Peter Krein - Tucson AZ, US
Tobin Jones - Tucson AZ, US

Assignee:

Ventana Medical Systems, Inc. - Tucson AZ

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

Methods and reagents for detecting high-risk human papilloma virus (HPV) DNA types in cells on a Pap smear which indicates the patient is at higher risk for cancer are described. The method differentiates high-risk from low-risk HPV DNA in cells, which indicates the patient's risk for cancer.

US Patent:

20060292615, Dec 28, 2006

Filed:

Jun 20, 2006

Appl. No.:

11/471971

Inventors:

Elizabeth Light - Gaithersburg MD, US

International Classification:

C12Q 1/68
C12N 9/02

US Classification:

435006000, 435188500

Abstract:

Compositions and methods are provided for detecting single copies of a gene in-situ using brightfield microscopy in detection of various target nucleic acid sequences, such as genes or nucleic acid of microorganisms in a cell. In particular, nucleic acid probes for various target nucleic acid sequences in a cell are utilized in conjunction with enzymes and their substrates to produce chromogenic composition associated with the target nucleic acid sequences in the cell.

US Patent:

58560890, Jan 5, 1999

Filed:

Jul 22, 1994

Appl. No.:

8/279315

Inventors:

Mary Ge Wang - Rockville MD
Albert Louis George - Gaithersburg MD
Elizabeth Sophia Light - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934
C07H 2104
C07H 2102

US Classification:

435 6

Abstract:

The present invention is directed to in situ hybridization methods using nucleic acid probes for single copy sequences for detecting chromosomal structural abnormalities in fixed tissue obtained from a patient suspected of having a chromosomal structural abnormality.

US Patent:

20220177951, Jun 9, 2022

Filed:

Nov 18, 2021

Appl. No.:

17/529405

Inventors:

- Gaithersburg MD, US
Madhvi Upender - Potomac MD, US
Elizabeth Light - Gaithersburg MD, US
Colyn Cargile Cain - Bethesda MD, US

Assignee:

NeoDiagnostix, Inc. - Gaithersburg MD

International Classification:

C12Q 1/6827
C12Q 1/6841
G06T 7/00
G06V 20/69
C12Q 1/6816

Abstract:

A method of screening for the presence and/or extent of a pathology in a subject, the pathology characterized by an abnormal chromosomal component in a cell of the subject, comprising the steps of: contacting a biological sample comprising cell nuclei from said subject with, one or more distinguishable labeled probes directed to at least one chromosomal sequence that characterizes the abnormality under conditions that promote hybridization of the one or more probes to the at least one sequence, automatically obtaining a representation of the one or more distinguishable labels hybridized to the chromosomal sequences, automatically analyzing the distribution and intensity of binding of the one or more labels in the representation to determine the presence and/or extent of an abnormal chromosomal component; and automatically reporting results of the analysis: wherein the steps are carried out without intervention by a human.

US Patent:

20190211382, Jul 11, 2019

Filed:

Mar 17, 2019

Appl. No.:

16/355796

Inventors:

- Gaithersburg MD, US
Madhvi Upender - Potomac MD, US
Elizabeth Light - Gaithersburg MD, US
Colyn Cargile Cain - Bethesda MD, US

Assignee:

NeoDiagnostix, Inc. - Gaithersburg MD

International Classification:

C12Q 1/6827
C12Q 1/6841
G06T 7/00
C12Q 1/6816
G06K 9/00

Abstract:

A method of screening for the presence and/or extent of a pathology in a subject, the pathology characterized by an abnormal chromosomal component in a cell of the subject, comprising the steps of: contacting a biological sample comprising cell nuclei from said subject with, one or more distinguishable labeled probes directed to at least one chromosomal sequence that characterizes the abnormality under conditions that promote hybridization of the one or more probes to the at least one sequence, automatically obtaining a representation of the one or more distinguishable labels hybridized to the chromosomal sequences, automatically analyzing the distribution and intensity of binding of the one or more labels in the representation to determine the presence and/or extent of an abnormal chromosomal component; and automatically reporting results of the analysis; wherein the steps are carried out without intervention by a human.

US Patent:

20170067123, Mar 9, 2017

Filed:

Nov 7, 2016

Appl. No.:

15/344875

Inventors:

- Gaithersburg MD, US
Madhvi Upender - Potomac MD, US
Elizabeth Light - Gaithersburg MD, US
Colyn Cain - Bethesda MD, US

International Classification:

C12Q 1/68
C12N 15/10

Abstract:

The invention provides for a diagnostic test to monitor cancer-specific genetic abnormalities to diagnose cervical cell disorders and predict which patients might progress to cancer. Genetic abnormalities are detected by identification in chromosomal copy number of chromosome 3 and 5 using FISH analysis of probes targeted to 3q and/or 5p.