Elizabeth K Meier

US Patent:

20210285014, Sep 16, 2021

Filed:

May 27, 2021

Appl. No.:

17/332160

Inventors:

- Emeryville CA, US
Sara da Luz Areosa Cleto - Emeryville CA, US
Cameron Coates - Oakland CA, US
Aaron Miller - Berkeley CA, US
Sharon Nademanee - Alameda CA, US
Melissa Netwal - Oakland CA, US
Kedar Patel - Fremont CA, US
Shawn Szyjka - Martinez CA, US
Philip Weyman - Alameda CA, US
Solomon Henry Stonebloom - Alameda CA, US
Colin Scott Maxwell - Emeryville CA, US
Elizabeth Lauren Meier - Oakland CA, US

International Classification:

C12N 15/90
C12N 15/65
C12N 1/20
C12N 1/16

Abstract:

The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

US Patent:

20200283780, Sep 10, 2020

Filed:

Mar 20, 2020

Appl. No.:

16/825683

Inventors:

- Emeryville CA, US
Sara da Luz Areosa CLETO - Emeryville CA, US
Cameron Coates - Oakland CA, US
Aaron Miller - Berkeley CA, US
Sharon Nademanee - Alameda CA, US
Melissa Netwal - Oakland CA, US
Kedar Patel - Fremont CA, US
Shawn Szyjka - Martinez CA, US
Philip Weyman - Alameda CA, US
Solomon Henry Stonebloom - Alameda CA, US
Colin Scott Maxwell - Emeryville CA, US
Elizabeth Lauren Meier - Oakland CA, US

International Classification:

C12N 15/70
C12N 15/113
C12N 9/22
C12N 15/81
C12N 15/80
C12N 15/77

Abstract:

The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

US Patent:

20200283802, Sep 10, 2020

Filed:

Mar 20, 2020

Appl. No.:

16/825710

Inventors:

- Emeryville CA, US
Sara da Luz Areosa Cleto - Emeryville CA, US
Cameron Coates - Oakland CA, US
Aaron Miller - Berkeley CA, US
Sharon Nademanee - Alameda CA, US
Melissa Netwal - Oakland CA, US
Kedar Patel - Fremont CA, US
Shawn Szyjka - Martinez CA, US
Philip Weyman - Alameda CA, US
Solomon Henry Stonebloom - Alameda CA, US
Colin Scott Maxwell - Emeryville CA, US
Elizabeth Lauren Meier - Emeryville CA, US

International Classification:

C12N 15/90
C12N 1/16
C12N 1/20
C12N 15/65

Abstract:

The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.