Ellen I Guthrie

US Patent:

6342365, Jan 29, 2002

Filed:

Feb 24, 1999

Appl. No.:

09/257153

Inventors:

Sharon T. Wong-Madden - Bellevue WA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12Q 134

US Classification:

435 18, 435 4, 435 74, 435 76, 435 772, 435183, 435200, 435201, 435207, 435208

Abstract:

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac1-X, Gal1-3R, Gal1-6R, Gal1-3R, Fuc1-2R, Fuc1-3R, Fuc1-4R, Man1-2R, Man1-3R, Man1-6R, Man1-4R, Xyl1-2R, Glc1-4R, and Gal1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

US Patent:

6358724, Mar 19, 2002

Filed:

Jun 18, 2001

Appl. No.:

09/883800

Inventors:

Sharon T. Wong-Madden - Bellevue WA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12N 938

US Classification:

435207, 435 4, 435 18, 435200, 435243, 4352521

Abstract:

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac1-X, Gal1-3R, Gal1-6R, Gal1-3R, Fuc-2R, Fuc1-3R, Fuc1-4R, Man1-2R, Man1-3R, Man1-6R, Man1-4R, Xyl1-2R, Glc1-4R, and Gal1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

US Patent:

7094563, Aug 22, 2006

Filed:

Nov 15, 2001

Appl. No.:

10/003136

Inventors:

Sharon T. Wong-Madden - Bellevue WA, US
Ellen P. Guthrie - Andover MA, US
David Landry - Essex MA, US
Christopher H. Taron - Champaign IL, US
Chudi Guan - Wenham MA, US
Phillips W. Robbins - Acton MA, US

Assignee:

New England Biolabs, Inc. - Ispwich MA

International Classification:

C12P 1/00
C12P 19/22
C12N 9/26
C12N 1/00
C12N 1/12

US Classification:

435 41, 435183, 435195, 435200, 435201, 435243, 4352521

Abstract:

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNacβ1-x, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R, Manβ1-4R, Xylβ1-2R, Glcβ1-4R, and Galβ1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.