Elon W Frampton

US Patent:

6660494, Dec 9, 2003

Filed:

Dec 19, 2001

Appl. No.:

10/021271

Inventors:

Günter Schabert - Goldach, CH
Lawrence Restaino - Chicago IL
Elon W. Frampton - DeKalb IL

Assignee:

Biosynth AG

International Classification:

C12Q 104

US Classification:

435 34, 435 29, 435 18, 435 21, 435 32, 43525231, 4352533, 435975, 548414

Abstract:

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C alkyl, such as methyl, ethyl, propyl and butyl while R , R , R , and R are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C alkyl groups, aminomethyl, hydroxy, C alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.