Eric James May

US Patent:

20120122094, May 17, 2012

Filed:

Feb 1, 2011

Appl. No.:

13/382509

Inventors:

Eric May - Chandler AZ, US
Alexandra Nagy - Oro Valley AZ, US
Jerome Kosmeder - Tucson AZ, US

International Classification:

G01N 21/64
C12Q 1/68
B82Y 15/00

US Classification:

435 611, 436501, 977774, 977902

Abstract:

Embodiments of a composition for stabilizing fluorescent signal of nanoparticles and methods for its use are disclosed. In some embodiments, the composition has a pH from 7 to 10 and includes borate, protein and/or protein hydrolysate, an amine, a preservative, and a nonionic surfactant. In particular embodiments, the amine is an N-ethanol substituted amine, such as ethanolamine, diethanolamine, triethanolamine, N-methyldiethanolamine, N,N-dimethylethanolamine, or a combination thereof. In some embodiments, a fluorescent particle solution, such as a quantum dot solution or quantum dot conjugate solution, is diluted in the composition and stored at 4 C. In certain embodiments, the fluorescence intensity of the diluted fluorescent particle remains substantially the same when stored at 4 C. for at least one month or at least three months. In particular embodiments, a diluted quantum dot conjugate is used to detect a hybridized probe or a protein antigen.

US Patent:

20120171668, Jul 5, 2012

Filed:

Dec 28, 2011

Appl. No.:

13/339066

Inventors:

Eric J. May - Chandler AZ, US
Adrian E. Murillo - Tucson AZ, US
Jerome W. Kosmeder - Tucson AZ, US

International Classification:

C12Q 1/68
G01N 33/536

US Classification:

435 61, 435 79

Abstract:

This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formulawhere R, R, R, and Rare independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; Rand/or Rcan be bound to Rto form a fused, aromatic ring system; Ris selected from a heteroatom-containing moiety; A is selected from, a carbon atom, a heteroatom, other than sulfur, and any combination thereof; n is 1-5, an enzyme, a specific binding moiety and a detectable moiety.

US Patent:

20130109019, May 2, 2013

Filed:

Jul 1, 2011

Appl. No.:

13/805978

Inventors:

Adrian E. Murillo - Tucson AZ, US
Jerome W. Kosmeder - Tucson AZ, US
Eric May - Chandler AZ, US
William Day - Tucson AZ, US
Mark Lefever - Oro Valley AZ, US
Anne M. Pedata - Tucson AZ, US
Christopher Bieniarz - Tucson AZ, US
Phillip Miller - Tucson AZ, US

International Classification:

C07D 277/54
C07C 237/20
C07D 271/12
C07D 233/90
C07D 243/00
C07D 241/52

US Classification:

435 611, 540517, 544355, 548126, 548185, 5483275, 564155, 435 28, 435 71, 435 79

Abstract:

Embodiments of hapten conjugates including a hapten, an optional linker, and a peroxidase-activatable aryl moiety are disclosed. In some embodiments, the peroxidase-activatable aryl moiety is tyramine or a tyramine derivative. Embodiments of methods for making and using the hapten conjugates also are disclosed. In particular embodiments, the hapten conjugates are used in a signal amplification assay. In certain embodiments, the hapten is an oxazole, a pyrazole, a thiazole, a benzofurazan, a triterpene, a urea, a thiourea other than a rhodamine thiourea, a nitroaryl other than dinitrophenyl or trinitrophenyl, a rotenoid, a cyclolignan, a heterobiaryl, an azoaryl, a benzodiazepine, or 7-diethylamino-3-carboxycoumarin. The hapten is coupled to the peroxidase-activatable aryl moiety directly or indirectly via a linker. In certain embodiments, the hapten conjugates are used in multiplexed assays.

US Patent:

20220306493, Sep 29, 2022

Filed:

Jun 9, 2022

Appl. No.:

17/836850

Inventors:

- Tucson AZ, US
Joseph M. Jakubowski - Georgetown TX, US
Eric May - Oro Valley AZ, US
Michael Otter - Tucson AZ, US
Taylor J. Shingler - Tucson AZ, US
Benjamin C. Stevens - Oro Valley AZ, US

International Classification:

C02F 1/32
C02F 1/72
G01N 1/31
G01N 35/00

Abstract:

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.

US Patent:

20220298028, Sep 22, 2022

Filed:

Jun 9, 2022

Appl. No.:

17/836724

Inventors:

- Tucson AZ, US
Joseph M. Jakubowski - Georgetown TX, US
Eric May - Oro Valley AZ, US
Michael Otter - Tucson AZ, US
Taylor J. Shingler - Tucson AZ, US
Benjamin C. Stevens - Oro Valley AZ, US

International Classification:

C02F 1/32
C02F 1/72
G01N 1/31
G01N 35/00

Abstract:

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.

US Patent:

20210349101, Nov 11, 2021

Filed:

Mar 17, 2021

Appl. No.:

17/203802

Inventors:

- Tucson AZ, US
Mark Lefever - Oro Valley AZ, US
Eric May - Oro Valley AZ, US

International Classification:

G01N 33/58
C09B 57/02
C09B 69/10

Abstract:

Disclosed herein are novel coumarin-based reagents, e.g. linkers, and conjugates including one or more of the disclosed coumarin-based reagents. In some embodiments, the presence of a coumarin moiety within the coumarin-based reagents enables detection of labels which are typically difficult to detect, e.g. certain haptens.

US Patent:

20200348215, Nov 5, 2020

Filed:

Jul 20, 2020

Appl. No.:

16/933658

Inventors:

- Tucson AZ, US
Brian D. Kelly - Tucson AZ, US
Eric J. May - Chandler AZ, US
Larry Morrison - Oro Valley AZ, US

International Classification:

G01N 1/30
C12Q 1/28
G01N 33/532
G01N 33/535
G01N 33/543
G01N 33/58

Abstract:

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

US Patent:

20190300392, Oct 3, 2019

Filed:

Jun 14, 2019

Appl. No.:

16/442225

Inventors:

- Tucson AZ, US
Joseph M. Jakubowski - Georgetown TX, US
Eric May - Oro Valley AZ, US
Michael Otter - Tucson AZ, US
Taylor J. Shingler - Tucson AZ, US
Benjamin C. Stevens - Oro Valley AZ, US

International Classification:

C02F 1/32
G01N 1/31
G01N 35/00
C02F 1/72

Abstract:

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.