Eric D May

US Patent:

20120122094, May 17, 2012

Filed:

Feb 1, 2011

Appl. No.:

13/382509

Inventors:

Eric May - Chandler AZ, US
Alexandra Nagy - Oro Valley AZ, US
Jerome Kosmeder - Tucson AZ, US

International Classification:

G01N 21/64
C12Q 1/68
B82Y 15/00

US Classification:

435 611, 436501, 977774, 977902

Abstract:

Embodiments of a composition for stabilizing fluorescent signal of nanoparticles and methods for its use are disclosed. In some embodiments, the composition has a pH from 7 to 10 and includes borate, protein and/or protein hydrolysate, an amine, a preservative, and a nonionic surfactant. In particular embodiments, the amine is an N-ethanol substituted amine, such as ethanolamine, diethanolamine, triethanolamine, N-methyldiethanolamine, N,N-dimethylethanolamine, or a combination thereof. In some embodiments, a fluorescent particle solution, such as a quantum dot solution or quantum dot conjugate solution, is diluted in the composition and stored at 4 C. In certain embodiments, the fluorescence intensity of the diluted fluorescent particle remains substantially the same when stored at 4 C. for at least one month or at least three months. In particular embodiments, a diluted quantum dot conjugate is used to detect a hybridized probe or a protein antigen.

US Patent:

20120171668, Jul 5, 2012

Filed:

Dec 28, 2011

Appl. No.:

13/339066

Inventors:

Eric J. May - Chandler AZ, US
Adrian E. Murillo - Tucson AZ, US
Jerome W. Kosmeder - Tucson AZ, US

International Classification:

C12Q 1/68
G01N 33/536

US Classification:

435 61, 435 79

Abstract:

This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formulawhere R, R, R, and Rare independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; Rand/or Rcan be bound to Rto form a fused, aromatic ring system; Ris selected from a heteroatom-containing moiety; A is selected from, a carbon atom, a heteroatom, other than sulfur, and any combination thereof; n is 1-5, an enzyme, a specific binding moiety and a detectable moiety.