Eric C Sigillo

US Patent:

8039272, Oct 18, 2011

Filed:

Apr 1, 2009

Appl. No.:

12/416570

Inventors:

Eric C. Sigillo - Marlborough MA, US
Jeffrey C. Chien - Wellesley MA, US
Mary C. Pomeroy - Framingham MA, US
Catherine A. Saltalamacchia - Winchester MA, US

Assignee:

Nova Biomedical - Waltham MA

International Classification:

G01N 33/553

US Classification:

436525, 436164, 436524, 436805

Abstract:

A concentrated and ligand-coated gold colloid and a method of preparing it. Also disclosed is a rapid biomolecule quantification assay involving the use of the concentrated gold colloid and an apparatus for performing the assay.

US Patent:

49313851, Jun 5, 1990

Filed:

Nov 21, 1988

Appl. No.:

7/275656

Inventors:

Elliott Block - Wellesley Hills MA
Izak Bahar - Chestnut Hill MA
Frank Cole - Stow MA
Cheryl A. Eaton - Southboro MA
Wendy Jones - Burlington MA
Eric Sigillo - Lawrence MA
Mary Coseo - Arlington MA
Nancy J. Cicia - Wakefield MA
L. Edward Cannon - Wayland MA
Walter Cantarow - Norwood MA

Assignee:

Hygeia Sciences, Incorporated - Newton MA

International Classification:

G01N 33535
G01N 33543
C12N 996
C12Q 128

US Classification:

435 7

Abstract:

Detection of bindable substances such as antibodies and antigens using enzyme linked immunosorbent assays having particular utility in home diagnostic applications. The preferred implementation of the invention is characterized by the steps of admixing a sample suspected of containing the bindable substance to be detected with an antibody-enzyme conjugate, immersing an antibody coated solid support into the mixture and then exposing the coated support to an activated chromogenic solution. The conjugate for use in the home diagnostic assay is preferably contained within a lyophilized mixture. The lyophilized mixture contains components which preserve the antibody-conjugate's reactivity and immunologic binding specificity even if the lyophilized mixture had been subjected to hot, humid environmental conditions. Active components in the lyophilized mixture include polyethylene glycol, sugars, and surfactant. The antibody coated support is treated with an improved blocking solution containing a blocking agent such as bovine serum albumin or milk protein in admixture with a sugar.

US Patent:

51418509, Aug 25, 1992

Filed:

Feb 7, 1990

Appl. No.:

7/475486

Inventors:

Francis X. Cole - Stow MA
Eric C. Sigillo - Methuen MA
Paul C. MacDonnell - Bedford MA
Nancy J. Cicia - Wakefield MA

Assignee:

Hygeia Sciences, Inc. - Newton MA

International Classification:

G01N 3353

US Classification:

436525

Abstract:

An immunoassay method comprising applying an aqueous solution containing the analyte antigen to one end of a multi-zoned test strip device such that the solution moves along the strip by capillary action. The zones are arranged so that the solution (a) first contacts and reconstitutes dry, diffusible labelled component comprising colloidal gold conjugated to an antibody specific for said analyte antigen and then (b) contacts and reconstitutes dry, diffusible biotinylated second antibody specific for said analyte antigen such that a diffusible, dispersed sandwich reaction product forms. The reaction product diffuses along the strip with the solution and into a zone containing capture component consisting of a latex and avidin complex which avidin collects the reaction product by means of reaction with its biotin moiety. Thus, gold particles are collected and concentrated in the detection zone for visual determination.