Eugene J Brown

US Patent:

7371207, May 13, 2008

Filed:

Apr 10, 2003

Appl. No.:

10/511064

Inventors:

Scott Alan Jelinsky - Acton MA, US
Heather Anne Harris - Phoenixville PA, US
Eugene Lee Brown - Newton MA, US
Mark James Evans - Radnor PA, US
Donald Edward Frail - Wildwood MO, US

Assignee:

Wyeth - Madison NJ

International Classification:

C40B 40/00

US Classification:

506 13, 435 6, 536 231, 536 2431, 536 235, 506 9

Abstract:

A plurality of genes modulated by estrogen or other agents, such as hormones or combinations of hormones, in various types of tissue is described. One embodiment of the disclosure relates to a plurality of genes, which demonstrates certain patterns of expression differing qualitatively or quantitatively, with and without exposure to estrogen and/or other hormone compositions. Methods of using these genes in identifying candidate agents that exert at least some of the biological effects of estrogen and/or other hormone, and pharmaceuticals and related therapies also is disclosed. The use of the plurality of genes in methods of monitoring, in gene chips and in kits also is disclosed.

US Patent:

20040265230, Dec 30, 2004

Filed:

Jan 6, 2004

Appl. No.:

10/751736

Inventors:

Robert Martinez - Roslindale MA, US
Eugene Brown - Newton Highlands MA, US
Wei Liu - Sudbury MA, US

International Classification:

A61K051/00
C12Q001/68
G01N033/574
A61K048/00

US Classification:

424/001490, 424/093200, 435/006000, 435/007230

Abstract:

Compositions, equipment, and methods for prognosing, diagnosing, preventing, or treating colon cancer. Colon cancer genes that are differentially expressed in colon cancer tissues relative to disease-free tissues are identified. Examples of these genes are illustrated in Tables 1-5. Colon cancer genes of the present invention and their encoded products can be used as markers or prophylactic or therapeutic agents for the detection or treatment of colon cancer.

US Patent:

20050266409, Dec 1, 2005

Filed:

Feb 4, 2004

Appl. No.:

10/770726

Inventors:

Eugene Brown - Newton Highlands MA, US
Wei Liu - Sudbury MA, US

Assignee:

Wyeth - Madison NJ

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50

US Classification:

435006000, 702020000

Abstract:

Compositions and methods for diagnosing, preventing, and treating cancers. In one embodiment, genes differentially expressed in colon, lung, breast and prostate cancer tissues relative to corresponding cancer-free tissues are identified. These genes or their products can be used as markers for the detection of respective cancers. Modulators of these genes or their products can be used for the treatment or prevention of respective cancers.

US Patent:

20060003958, Jan 5, 2006

Filed:

May 11, 2005

Appl. No.:

11/128061

Inventors:

Mark Melville - Melrose MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester MA, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US

International Classification:

A61K 48/00
C07K 14/47
C12Q 1/68
C07H 21/04
C12N 5/06

US Classification:

514044000, 435006000, 435069100, 435358000, 530350000, 536023500

Abstract:

The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.

US Patent:

20060010513, Jan 12, 2006

Filed:

May 11, 2005

Appl. No.:

11/128049

Inventors:

Mark Melville - Melrose MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester NH, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US

International Classification:

A01H 1/00
C12N 15/82

US Classification:

800278000

Abstract:

The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.

US Patent:

20070270331, Nov 22, 2007

Filed:

Mar 13, 2007

Appl. No.:

11/717426

Inventors:

Stephen Berasi - Arlington MA, US
Christine Huard - Somerville MA, US
Dongmei Li - Wayland MA, US
Heather Shih - Andover MA, US
Ying Sun - Maynard MA, US
Janet Paulsen - Londonderry NH, US
Eugene Brown - Newton Highlands MA, US
Ruth Gimeno - Wellesley MA, US
Robert Martinez - Carlisle MA, US

International Classification:

A61K 38/02
A61K 31/7052
A61P 3/08
C12N 5/02

US Classification:

514002000, 435375000, 514044000

Abstract:

The present invention provides methods of affecting gluconeogenesis by altering the levels or activity of DUSP4 and/or EGR1. Pharmaceutical compositions comprising or encoding modulators of gluconeogenesis are also disclosed.

US Patent:

20090022731, Jan 22, 2009

Filed:

Aug 16, 2007

Appl. No.:

11/893933

Inventors:

David Johannes von Schack - Arlington MA, US
Matthew James Whitters - Hudson MA, US
Mary Collins - Natick MA, US
Eugene Lee Brown - Newton MA, US

Assignee:

Wyeth - Madison NJ

International Classification:

C07K 16/18
C07K 16/40
A61K 39/395
A61P 19/02

US Classification:

4241451, 435 6, 435455, 435375, 5303871, 5303882, 53038826, 4241581, 4241461

Abstract:

The invention features methods and compositions benefiting from differential gene expression observed in arthritis-associated B cells.

US Patent:

20100029500, Feb 4, 2010

Filed:

Jun 26, 2009

Appl. No.:

12/492832

Inventors:

MARK W. MELVILLE - MELROSE MA, US
TIMOTHY S. CHARLEBOIS - ANDOVER MA, US
WILLIAM M. MOUNTS - ANDOVER MA, US
LOUANE E. HANN - BOSTON MA, US
MARTIN S. SINACORE - ANDOVER MA, US
MARK W. LEONARD - MANCHESTER NH, US
EUGENE L. BROWN - NEWTON HIGHLANDS MA, US
CHRISTOPHER P. MILLER - ARLINTON MA, US
GENE W. LEE - CHELMSFORD MA, US

Assignee:

WYETH - MADISON NJ

International Classification:

C40B 30/04
C40B 40/06

US Classification:

506 9, 506 16

Abstract:

The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.