Eugene W Brown

US Patent:

6410229, Jun 25, 2002

Filed:

Dec 14, 1998

Appl. No.:

09/212004

Inventors:

David J. Lockhart - Santa Clara CA
Eugene L. Brown - Newton Highlands MA
Gordon G. Wong - Brookline MA
Mark S. Chee - Palo Alto CA
Thomas R. Gingeras - Encinitas CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 536 232, 536 235, 536 253

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm , and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

US Patent:

20020012940, Jan 31, 2002

Filed:

Aug 22, 2001

Appl. No.:

09/935365

Inventors:

David Lockhart - Santa Clara CA, US
Eugene Brown - Newton Highlands MA, US
Gordon Wong - Brookline MA, US
Mark Chee - Palo Alto CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

US Patent:

20050158746, Jul 21, 2005

Filed:

Nov 23, 2004

Appl. No.:

10/998518

Inventors:

David Lockhart - Santa Clara CA, US
Eugene Brown - Newton Highlands MA, US
Gordon Wong - Brookline MA, US
Mark Chee - Palo Alto CA, US
Thomas Gingeras - Encinitas CA, US

Assignee:

Affymetrix Inc. - Santa Clara CA

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

US Patent:

60401389, Mar 21, 2000

Filed:

Sep 15, 1995

Appl. No.:

8/529115

Inventors:

David J. Lockhart - Santa Clara CA
Eugene L. Brown - Newton Highlands MA
Gordon G. Wong - Brookland NY
Mark S. Chee - Palo Alto CA
Thomas R. Gingeras - Encinitas CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168
C07H 2104
C07H 2102

US Classification:

435 6

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm. sup. 2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.