Eugene Larry Brown

US Patent:

6927032, Aug 9, 2005

Filed:

Jan 28, 2003

Appl. No.:

10/353792

Inventors:

David J. Lockhart - Del Mar CA, US
Eugene L. Brown - Newton Highlands MA, US
Gordon G. Wong - Brookline MA, US
Mark Chee - Palo Alto CA, US
Thomas R. Gingeras - Encinitas CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q001/68
C12P019/34
C12M001/34
C07H021/02
C07H021/04

US Classification:

435 6, 435 911, 4352811, 4352812, 536 231, 536 243

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

US Patent:

20050202500, Sep 15, 2005

Filed:

Apr 28, 2005

Appl. No.:

11/118974

Inventors:

David Lockhart - Del Mar CA, US
Eugene Brown - Newton Highlands MA, US
Gordon Wong - Brookline MA, US
Mark Chee - Palo Alto CA, US
Thomas Gingeras - Encinitas CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50
C12P019/34

US Classification:

435006000, 435091200, 702020000

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.