Ewald M Wondrak

US Patent:

6555382, Apr 29, 2003

Filed:

Aug 3, 2001

Appl. No.:

09/920938

Inventors:

Ewald M. Wondrak - Frederick MD 21701

International Classification:

G01N 3348

US Classification:

436 86, 436 15, 436164

Abstract:

The present invention provides an improved solution for staining proteins in and/or on a solid matrix or support. Typically, a protein-containing gel or membrane may be washed in a hot solution of water for about five minutes, stained in a hot solution of COOMASSIE brilliant blue dye in dilute aqueous mineral acid for about five minutes, and then rinsed in water. The washing and/or staining steps may be performed by placing the gel in a wash and/or staining solution, respectively, heating the solution in which the gel is placed to boiling in a microwave oven, and incubating the gel in the solution for about five minutes. The entire procedure can be performed in a little over ten minutes, which represents an enormous time savings over existing methods.

US Patent:

63197200, Nov 20, 2001

Filed:

Oct 13, 1999

Appl. No.:

9/417298

Inventors:

Ewald M. Wondrak - Frederick MD

International Classification:

G01N 3348

US Classification:

436 86

Abstract:

The present invention provides an improved method for staining proteins in and/or on a solid matrix or support. Typically, a protein-containing gel or membrane may be washed in a hot solution of water for about five minutes, stained in a hot solution of COOMASSIE brilliant blue dye in dilute aqueous mineral acid for about five minutes, and then rinsed in water. The washing and/or staining steps may be performed by placing the gel in a wash and/or staining solution, respectively, heating the solution in which the gel is placed to boiling in a microwave oven, and incubating the gel in the solution for about five minutes. The entire procedure can be performed in a little over ten minutes, which represents an enormous time savings over existing methods.