Skills:
protein sequencing, peptide synthesis, RP-HPLC, AKTA explorer FPLC, ion exchange chromatography, size-exclusion chromatography, affinity chromatography, over-expression and purification of proteins in E. coli, insect cell lines and Leishmania tarentolae cell lines, tandem affinity purification (TAP) of protein complex, SDS-PAGE protein gels, 2D gel, blue native gel, Western blotting, Multi-PID, MALDI-TOF, ESI and LC-ESI, Electro Microcopy, Fluorescence Microscopy, preparation and affinity purification of polyclonal or monoclonal antibody, ELISA, immunoprecipitation. Recombinant DNA techniques, PCR, real-time PCR, DNA/RNA isolation, southern blotting and northern blotting, RNAi, next generation DNA sequencing (illumina platform), RNA-seq.