Skills:
Molecular cloning techniques: RNA and DNA isolation, DNA-mediated transformation, plasmid amplification and purification, cDNA synthesis, gene subcloning. Realtime PCR (SYBGreen assay and Taqman technology), primer and probe design Gene transfection: Liposome method and Amaxa Nucleofector technologies Gene sequencing Gene transcription and translation in vitro Gene knock down using siRNA and MicroRNA (GIPZ Lentiviral shRNAmir and TRIPZ inducible shRNAmir ) Protein detection: Western blot, ELISA, immunoprecipitation, immunocytochemistry DNA-protein interaction: Gel shift (EMSA, radioactive and biotin labeled), chromatin immunoprecipitation (CHIP) from cell lines, primary neurons, primary hepatocyte and 2 tissues, CHIP-SEQ validation FISH: Probe preparation and hybridization Cell culture: Primary cell culture from rats: Hippocampal neurons, Cerebellar granule cells; Cell lines: PC12, AtT20, Htt-20, Cos7, Hek293, HUH7, NIH3T3, HUVEC, Hela, DLD-1, SW480, MCF-10A etc; Single cell cloning; Establishment of human colon cancer cell lines from tumor xenograft on nude mice. Animal handling: Mouse and rat including KO mice breeding and nude mice assay